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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F12%3A00057288%21RIV13-GA0-14310___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans was investigated. The apparent Michaelis constant for ferric iron was 18.6 mM. An absence of anaerobic ferric iron reduction ability was observed in bacteria maintained on elemental sulfur for an extended period of time. Upon transition from ferrous iron to elemental sulfur medium, the cells exhibited similar kinetic characteristics of ferric iron reduction under anaerobic conditions to those of cells that were originally maintained on ferrous iron. Nevertheless, a total loss of anaerobic ferric iron reduction ability after the sixth passage in elemental sulfur medium was demonstrated. The first proteomic screening of total cell lysates of anaerobically incubated bacteria resulted in the detection of 1599 protein spots in the master two-dimensional electrophoresis gel. Elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans was investigated. The apparent Michaelis constant for ferric iron was 18.6 mM. An absence of anaerobic ferric iron reduction ability was observed in bacteria maintained on elemental sulfur for an extended period of time. Upon transition from ferrous iron to elemental sulfur medium, the cells exhibited similar kinetic characteristics of ferric iron reduction under anaerobic conditions to those of cells that were originally maintained on ferrous iron. Nevertheless, a total loss of anaerobic ferric iron reduction ability after the sixth passage in elemental sulfur medium was demonstrated. The first proteomic screening of total cell lysates of anaerobically incubated bacteria resulted in the detection of 1599 protein spots in the master two-dimensional electrophoresis gel.
dcterms:title
Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS
skos:prefLabel
Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS
skos:notation
RIV/00216224:14310/12:00057288!RIV13-GA0-14310___
n13:predkladatel
n14:orjk%3A14310
n3:aktivita
n11:Z n11:P
n3:aktivity
P(GA525/08/0697), Z(MSM0021622413), Z(MSM0021622415)
n3:cisloPeriodika
3
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n12:6890091 n12:4568567 n12:6563902 n12:1401521 n12:7777388 n12:3331091 n12:2503786
n3:druhVysledku
n18:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
144657
n3:idVysledku
RIV/00216224:14310/12:00057288
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Acidithiobacillus; Anaerobic sulfur oxidation; Ferric iron reduction; Proteomics; MS analysis
n3:klicoveSlovo
n6:Ferric%20iron%20reduction n6:MS%20analysis n6:Anaerobic%20sulfur%20oxidation n6:Proteomics n6:Acidithiobacillus
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[684571D252F4]
n3:nazevZdroje
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
n3:obor
n19:CE
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n15:GA525%2F08%2F0697
n3:rokUplatneniVysledku
n10:2012
n3:svazekPeriodika
101
n3:tvurceVysledku
Potěšil, David Zdráhal, Zbyněk Černá, Hana Kučera, Jiří Mandl, Martin Janiczek, Oldřich Bouchal, Pavel
n3:wos
000300314200010
n3:zamer
n16:MSM0021622415 n16:MSM0021622413
s:issn
0003-6072
s:numberOfPages
13
n21:doi
10.1007/s10482-011-9670-2
n20:organizacniJednotka
14310