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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F11%3A00049467%21RIV12-GA0-14310___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases. The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases.
dcterms:title
Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity
skos:prefLabel
Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity
skos:notation
RIV/00216224:14310/11:00049467!RIV12-GA0-14310___
n3:predkladatel
n14:orjk%3A14310
n4:aktivita
n12:P n12:Z
n4:aktivity
P(GA301/09/1115), P(GD204/08/H054), P(IAA501630801), Z(MSM0021622415)
n4:dodaniDat
n17:2012
n4:domaciTvurceVysledku
n10:9263942 n10:7513046 n10:6659446
n4:druhVysledku
n9:O
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
189299
n4:idVysledku
RIV/00216224:14310/11:00049467
n4:jazykVysledku
n15:eng
n4:klicovaSlova
cathepsin D; cell migration; breast cancer
n4:klicoveSlovo
n11:cathepsin%20D n11:cell%20migration n11:breast%20cancer
n4:kontrolniKodProRIV
[B97B7D39BD9B]
n4:obor
n18:EB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n8:GA301%2F09%2F1115 n8:GD204%2F08%2FH054 n8:IAA501630801
n4:rokUplatneniVysledku
n17:2011
n4:tvurceVysledku
Knopfová, Lucia Jančeková, Blanka Beneš, Petr Šmarda, Jan
n4:zamer
n5:MSM0021622415
n19:organizacniJednotka
14310