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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F10%3A00047719%21RIV11-GA0-14310___
rdf:type
n17:Vysledek skos:Concept
dcterms:description
Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for the description of their genomic structure. Here we have updated a multiplex PCR strategy for the complex characterization of S. aureus phages of the family Siphoviridae. Based on the comparative genomic analysis of the available phage sequences, a multilocus PCR strategy for typing the major modules of the phage genome was designed. The genomic modules were classified on the basis of the genes for integrase, anti-repressor, replication proteins, dUTPase, portal protein, tail appendices and endolysin corresponding to main functional modules. The nine PCR assays designed for the above sequences were shown to be capable to identify the bacteriophage gene pool present both in the phage and bacterial genomes and their mosaic structure. Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for the description of their genomic structure. Here we have updated a multiplex PCR strategy for the complex characterization of S. aureus phages of the family Siphoviridae. Based on the comparative genomic analysis of the available phage sequences, a multilocus PCR strategy for typing the major modules of the phage genome was designed. The genomic modules were classified on the basis of the genes for integrase, anti-repressor, replication proteins, dUTPase, portal protein, tail appendices and endolysin corresponding to main functional modules. The nine PCR assays designed for the above sequences were shown to be capable to identify the bacteriophage gene pool present both in the phage and bacterial genomes and their mosaic structure.
dcterms:title
Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure
skos:prefLabel
Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure
skos:notation
RIV/00216224:14310/10:00047719!RIV11-GA0-14310___
n3:aktivita
n11:R n11:S n11:P n11:V n11:Z
n3:aktivity
P(GA310/09/0459), R, S, V, Z(MSM0021622415)
n3:cisloPeriodika
9
n3:dodaniDat
n10:2011
n3:domaciTvurceVysledku
n12:3440656 n12:6303293 n12:4186672 n12:5051207 n12:9056157
n3:druhVysledku
n7:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
273126
n3:idVysledku
RIV/00216224:14310/10:00047719
n3:jazykVysledku
n8:eng
n3:klicovaSlova
bacteriophage; Staphylococcus aureus; Siphoviridae; molecular typing; PCR; modular genome; evolution
n3:klicoveSlovo
n5:Siphoviridae n5:modular%20genome n5:Staphylococcus%20aureus n5:bacteriophage n5:evolution n5:molecular%20typing n5:PCR
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[373D3DD510DE]
n3:nazevZdroje
Environmental Microbiology
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n13:GA310%2F09%2F0459
n3:rokUplatneniVysledku
n10:2010
n3:svazekPeriodika
12
n3:tvurceVysledku
Goerke, Christiane Doškař, Jiří Rájová, Jana Holochová, Pavla Pantůček, Roman Růžičková, Vladislava
n3:wos
000281556900013
n3:zamer
n4:MSM0021622415
s:issn
1462-2912
s:numberOfPages
12
n19:organizacniJednotka
14310