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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F10%3A00045193%21RIV11-MSM-14310___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
The human gastrointestinal tract contains a diverse microbial community. Among them lactobacilli are important members of the natural intestinal microbiota playing a beneficial role in balancing of the intestinal environment. A collection of 51 Lactobacillus isolates originating from children intestinal mucous tissues has been studied. Analysed biopsy samples were retrieved during a routine endoscopic examination of different parts of the large intestine in patients revealing various disorders. A combined approach, which included automated ribotyping and MALDI-TOF MS analysis, was applied for identification of isolated Lactobacillus spp. in order to compare the discriminatory power of these methods. Automated ribotyping using the RiboPrinter Microbial Characterization System (DuPont Qualicon) assigned 38 lactobacilli to the species level: Lactobacillus salivarius (29 isolates), Lactobacillus paracasei subsp. paracasei (7) and Lactobacillus rhamnosus (2). The human gastrointestinal tract contains a diverse microbial community. Among them lactobacilli are important members of the natural intestinal microbiota playing a beneficial role in balancing of the intestinal environment. A collection of 51 Lactobacillus isolates originating from children intestinal mucous tissues has been studied. Analysed biopsy samples were retrieved during a routine endoscopic examination of different parts of the large intestine in patients revealing various disorders. A combined approach, which included automated ribotyping and MALDI-TOF MS analysis, was applied for identification of isolated Lactobacillus spp. in order to compare the discriminatory power of these methods. Automated ribotyping using the RiboPrinter Microbial Characterization System (DuPont Qualicon) assigned 38 lactobacilli to the species level: Lactobacillus salivarius (29 isolates), Lactobacillus paracasei subsp. paracasei (7) and Lactobacillus rhamnosus (2).
dcterms:title
Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
skos:prefLabel
Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
skos:notation
RIV/00216224:14310/10:00045193!RIV11-MSM-14310___
n3:aktivita
n9:P n9:Z
n3:aktivity
P(2B08068), P(LC06034), Z(MSM0021622415), Z(MSM0021622416)
n3:dodaniDat
n10:2011
n3:domaciTvurceVysledku
n6:9410139 n6:4568567 n6:8113637 n6:9384839 n6:3787672
n3:druhVysledku
n11:O
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
262702
n3:idVysledku
RIV/00216224:14310/10:00045193
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Identification; Lactobacillus; MALDI-TOF MS; RiboPrinter
n3:klicoveSlovo
n7:Identification n7:MALDI-TOF%20MS n7:Lactobacillus n7:RiboPrinter
n3:kontrolniKodProRIV
[16021D742CD9]
n3:obor
n8:EE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n13:2B08068 n13:LC06034
n3:rokUplatneniVysledku
n10:2010
n3:tvurceVysledku
Švec, Pavel Sedláček, Ivo Dráb, Vladimír Zdráhal, Zbyněk Šedo, Ondrej Teshim, Andrea
n3:zamer
n15:MSM0021622415 n15:MSM0021622416
n17:organizacniJednotka
14310