This HTML5 document contains 58 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n19http://localhost/temp/predkladatel/
n15http://linked.opendata.cz/resource/domain/vavai/projekt/
n9http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n16http://linked.opendata.cz/ontology/domain/vavai/
n5http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
skoshttp://www.w3.org/2004/02/skos/core#
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n17http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14310%2F10%3A00045039%21RIV13-MSM-14310___/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n6http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F10%3A00045039%21RIV13-MSM-14310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 is a bacterial enzyme that shows catalytic activity for the hydrolytic degradation of the highly toxic industrial pollutant 1,2,3-trichloropropane (TCP). Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y), DhaA14 (I135F) and DhaA15 (C176Y + I135F) were constructed in order to study the functional relevance of the tunnels connecting the buried active site of the protein with the surrounding solvent. All three protein variants were crystallized using the sitting-drop vapour-diffusion technique. The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 is a bacterial enzyme that shows catalytic activity for the hydrolytic degradation of the highly toxic industrial pollutant 1,2,3-trichloropropane (TCP). Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y), DhaA14 (I135F) and DhaA15 (C176Y + I135F) were constructed in order to study the functional relevance of the tunnels connecting the buried active site of the protein with the surrounding solvent. All three protein variants were crystallized using the sitting-drop vapour-diffusion technique.
dcterms:title
Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.
skos:prefLabel
Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.
skos:notation
RIV/00216224:14310/10:00045039!RIV13-MSM-14310___
n4:aktivita
n8:Z n8:I n8:S n8:P
n4:aktivity
I, P(GA310/09/1407), P(LC06010), S, Z(AV0Z40500505), Z(AV0Z60870520), Z(MSM0021622412), Z(MSM0021622413), Z(MSM6007665808)
n4:cisloPeriodika
66
n4:dodaniDat
n6:2013
n4:domaciTvurceVysledku
n9:2950103 n9:4694031 n9:2070103 Stsiapanava, Alena n9:1030175
n4:druhVysledku
n13:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
248048
n4:idVysledku
RIV/00216224:14310/10:00045039
n4:jazykVysledku
n18:eng
n4:klicovaSlova
haloalkane dehalogenase DhaA; Rhodococcus rhodochrous NCIMB 13064; hydrolytic degradation; 1.2.3-trichloropropane (TCP)
n4:klicoveSlovo
n10:Rhodococcus%20rhodochrous%20NCIMB%2013064 n10:hydrolytic%20degradation n10:haloalkane%20dehalogenase%20DhaA n10:1.2.3-trichloropropane%20%28TCP%29
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[991769FFFFC0]
n4:nazevZdroje
ACTA CRYSTALLOGRAPHICA D
n4:obor
n14:CE
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
7
n4:projekt
n15:LC06010 n15:GA310%2F09%2F1407
n4:rokUplatneniVysledku
n6:2010
n4:svazekPeriodika
66
n4:tvurceVysledku
Gavira, J. Stsiapanava, Alena Damborský, Jiří Dohnálek, J. Kutá-Smatanová, Ivana Koudeláková, Táňa Kutý, Michal
n4:wos
000281635500002
n4:zamer
n5:AV0Z40500505 n5:MSM6007665808 n5:MSM0021622412 n5:MSM0021622413 n5:AV0Z60870520
s:issn
0907-4449
s:numberOfPages
8
n19:organizacniJednotka
14310