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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F10%3A00044833%21RIV11-GA0-14310___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes. This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes.
dcterms:title
On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection
skos:prefLabel
On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection
skos:notation
RIV/00216224:14310/10:00044833!RIV11-GA0-14310___
n3:aktivita
n8:Z n8:P
n3:aktivity
P(GAP206/10/0057), P(LC06023), Z(MSM0021622413)
n3:cisloPeriodika
19
n3:dodaniDat
n9:2011
n3:domaciTvurceVysledku
n15:4767187 n15:1188607 n15:5235979 n15:2437694
n3:druhVysledku
n16:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
276993
n3:idVysledku
RIV/00216224:14310/10:00044833
n3:jazykVysledku
n17:eng
n3:klicovaSlova
CE; Cytochrome P450; Dextromethorphan; Metabolism
n3:klicoveSlovo
n10:CE n10:Metabolism n10:Cytochrome%20P450 n10:Dextromethorphan
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[5EA43AE25B7E]
n3:nazevZdroje
Electrophoresis
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:projekt
n5:GAP206%2F10%2F0057 n5:LC06023
n3:rokUplatneniVysledku
n9:2010
n3:svazekPeriodika
31
n3:tvurceVysledku
Zeisbergerová, Marta Hoogmartens, Jos Glatz, Zdeněk Řemínek, Roman Mádr, Aleš Van Schepdael, Ann
n3:zamer
n13:MSM0021622413
s:issn
0173-0835
s:numberOfPages
7
n12:organizacniJednotka
14310