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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F10%3A00043613%21RIV11-GA0-14310___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several new types of regulation. The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several new types of regulation.
dcterms:title
Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach
skos:prefLabel
Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach
skos:notation
RIV/00216224:14310/10:00043613!RIV11-GA0-14310___
n3:aktivita
n14:Z n14:S n14:P
n3:aktivity
P(GP203/07/P471), S, Z(MSM0021622413), Z(MSM0021622415)
n3:cisloPeriodika
6
n3:dodaniDat
n7:2011
n3:domaciTvurceVysledku
n6:3087719 n6:5589622 n6:3818780 n6:2503786 n6:4568567 n6:2474115 n6:9410139
n3:druhVysledku
n4:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
294406
n3:idVysledku
RIV/00216224:14310/10:00043613
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Paracoccus denitrificans; FNR transcription regulator; Respiratory switch; Two-dimensional gel electrophoresis; Proteome
n3:klicoveSlovo
n5:Respiratory%20switch n5:Two-dimensional%20gel%20electrophoresis n5:Paracoccus%20denitrificans n5:Proteome n5:FNR%20transcription%20regulator
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[F1225EFE18E3]
n3:nazevZdroje
Biochimica et Biophysica Acta - Proteins and Proteomics
n3:obor
n9:CE
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
8
n3:projekt
n16:GP203%2F07%2FP471
n3:rokUplatneniVysledku
n7:2010
n3:svazekPeriodika
1804
n3:tvurceVysledku
Kučera, Igor van Spanning, Rob Struhárová, Iva Bouchal, Pavel Budinská, Eva Vyhlídalová, Tereza Šedo, Ondrej Zdráhal, Zbyněk
n3:zamer
n18:MSM0021622413 n18:MSM0021622415
s:issn
1570-9639
s:numberOfPages
8
n19:organizacniJednotka
14310