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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F10%3A00043372%21RIV11-GA0-14310___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Ascaridoid nematode species Brevimulticaecum heterotis obtained by parasitological dissection from the African bonytongue, Heterotis niloticus (Osteoglossidae) collected in Senegal has been chosen for studies by scanning electron microscope to redescribe its external morphology. Scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were used for the study. For SEM, specimens were fixed with hot formalin. They were dehydrated, critical-point dried, mounted on stubs and coated with gold. For ESEM, fixed specimens were hydrated with water; desiccation was not applied. Quanta 3D FEG and Quanta 250 FEG in SEM and ESEM mode were used. Combination of both techniques brings new level to nematode investigation. While SEM remains unsubstitutable method for study of nematode morphology due to its ease of use, ESEM is an unexceptionable instrument when there is only one unique specimen that cannot be destroyed or must remain available for molecular studies. Ascaridoid nematode species Brevimulticaecum heterotis obtained by parasitological dissection from the African bonytongue, Heterotis niloticus (Osteoglossidae) collected in Senegal has been chosen for studies by scanning electron microscope to redescribe its external morphology. Scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were used for the study. For SEM, specimens were fixed with hot formalin. They were dehydrated, critical-point dried, mounted on stubs and coated with gold. For ESEM, fixed specimens were hydrated with water; desiccation was not applied. Quanta 3D FEG and Quanta 250 FEG in SEM and ESEM mode were used. Combination of both techniques brings new level to nematode investigation. While SEM remains unsubstitutable method for study of nematode morphology due to its ease of use, ESEM is an unexceptionable instrument when there is only one unique specimen that cannot be destroyed or must remain available for molecular studies.
dcterms:title
COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE
skos:prefLabel
COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE
skos:notation
RIV/00216224:14310/10:00043372!RIV11-GA0-14310___
n3:aktivita
n15:Z n15:S n15:P
n3:aktivity
P(GD526/09/H025), P(LC522), S, Z(MSM0021622416)
n3:dodaniDat
n10:2011
n3:domaciTvurceVysledku
n7:7196334 n7:7490917
n3:druhVysledku
n5:O
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
251433
n3:idVysledku
RIV/00216224:14310/10:00043372
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Nematoda; ESEM; SEM; nematode; Heterotis niloticus; Brevimulticaecum heterotis
n3:klicoveSlovo
n4:SEM n4:nematode n4:Nematoda n4:ESEM n4:Brevimulticaecum%20heterotis n4:Heterotis%20niloticus
n3:kontrolniKodProRIV
[533D1E5BE619]
n3:obor
n12:EG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n11:GD526%2F09%2FH025 n11:LC522
n3:rokUplatneniVysledku
n10:2010
n3:tvurceVysledku
Baruš, Vlastimil Wandrol, Petr Mašová, Šárka
n3:zamer
n16:MSM0021622416
n14:organizacniJednotka
14310