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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F10%3A00040598%21RIV11-MSM-14310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters. Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.
dcterms:title
Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering
skos:prefLabel
Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering
skos:notation
RIV/00216224:14310/10:00040598!RIV11-MSM-14310___
n3:aktivita
n11:Z n11:P
n3:aktivity
P(IAA401630901), P(LC06010), Z(MSM0021622412)
n3:cisloPeriodika
35
n3:dodaniDat
n10:2011
n3:domaciTvurceVysledku
Sato, Yukari n5:1713140 n5:2070103 Nagata, Yuji n5:6084559 n5:3599019 n5:2807602 n5:1030175 n5:5771943 n5:7612656
n3:druhVysledku
n14:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
257086
n3:idVysledku
RIV/00216224:14310/10:00040598
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Enantioselectivity; Haloalkane Dehalogenases; protein engineering
n3:klicoveSlovo
n4:protein%20engineering n4:Haloalkane%20Dehalogenases n4:Enantioselectivity
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[2B640E4C6038]
n3:nazevZdroje
Angewandte Chemie International Edition
n3:obor
n19:CE
n3:pocetDomacichTvurcuVysledku
10
n3:pocetTvurcuVysledku
15
n3:projekt
n9:LC06010 n9:IAA401630901
n3:rokUplatneniVysledku
n10:2010
n3:svazekPeriodika
49
n3:tvurceVysledku
Senda, T. Brezovský, Jan Jeřábek, Petr Sato, Yukari Chaloupková, Radka Natsume, Rio Damborský, Jiří Štěpánková, Veronika Nagata, Yuji Koudeláková, Táňa Leewen, J.G.E. Janssen, D,B. Prokop, Zbyněk Mozga, Tomáš Florian, J.
n3:wos
000281316000011
n3:zamer
n6:MSM0021622412
s:issn
1433-7851
s:numberOfPages
5
n12:organizacniJednotka
14310