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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F09%3A00037430%21RIV10-MSM-14310___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 +/- 0.1) degrees of Celsius): adenine (pK_HL = 9.65 +/- 0.04, pK_H2L = 4.18 +/- 0.04), adenosine (pK_H2L = 3.59 +/- 0.05), cytosine (pK_H2L = 4.56 +/- 0.01), cytidine (pK_H2L = 4.16 +/- 0.02). In addition, thermodynamic parameters for bases were calculated. Acidobasic behavior of oligonucleotides ((CAC)3, A3C6, C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C-C+) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature. Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 +/- 0.1) degrees of Celsius): adenine (pK_HL = 9.65 +/- 0.04, pK_H2L = 4.18 +/- 0.04), adenosine (pK_H2L = 3.59 +/- 0.05), cytosine (pK_H2L = 4.56 +/- 0.01), cytidine (pK_H2L = 4.16 +/- 0.02). In addition, thermodynamic parameters for bases were calculated. Acidobasic behavior of oligonucleotides ((CAC)3, A3C6, C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C-C+) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature.
dcterms:title
Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine
skos:prefLabel
Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine
skos:notation
RIV/00216224:14310/09:00037430!RIV10-MSM-14310___
n3:aktivita
n14:S n14:P
n3:aktivity
P(LC06035), S
n3:cisloPeriodika
6
n3:dodaniDat
n5:2010
n3:domaciTvurceVysledku
n4:2200643 n4:2945541 n4:6359922 n4:9260501 n4:9007563 n4:8198020 n4:3790185 n4:1455990
n3:druhVysledku
n16:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
342909
n3:idVysledku
RIV/00216224:14310/09:00037430
n3:jazykVysledku
n13:eng
n3:klicovaSlova
oligonucleotides; adenine; cytosine; protonation; thermodynamics; chemometrics
n3:klicoveSlovo
n8:chemometrics n8:cytosine n8:protonation n8:oligonucleotides n8:thermodynamics n8:adenine
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[8A8F2F54BDA2]
n3:nazevZdroje
Chem. Papers
n3:obor
n10:CB
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
8
n3:projekt
n15:LC06035
n3:rokUplatneniVysledku
n5:2009
n3:svazekPeriodika
63
n3:tvurceVysledku
Lubal, Přemysl Táborský, Petr Dospivová, Dana Trnková, Libuše Pilařová, Iveta Vojtylová, Terézia Farková, Marta Třísková, Olga
s:issn
0366-6352
s:numberOfPages
7
n17:organizacniJednotka
14310