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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F09%3A00029998%21RIV10-MSM-14310___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Two-component systems belong to important sensing / response mechanisms in higher plants. AHP proteins interact with histidine kinase receptors as well as with response regulators, and therefore link the signal recognition by membrane sensors with regulation of transcription via nuclear-located transcription factors that mediate plant responses to the environmental stimuli. In our study the interactions of CKI1 receiver domain with all six AHP proteins were analyzed in vivo. Using the yeast two-hybrid system we revealed that CKI1 receiver domain interacts with AHP2, AHP3 and AHP5, but not with AHP1, AHP4 and AHP6. In addition, bimolecular fluorescence complementation analysis (BiFC) was carried out in transiently transformed tobacco leaves and the interaction pattern was confirmed. Combining these two methods for analysis of protein-protein interactions in vivo we gained a rapid insight into the specificity of elements involved in a signalling cascade downstream of CKI1 Two-component systems belong to important sensing / response mechanisms in higher plants. AHP proteins interact with histidine kinase receptors as well as with response regulators, and therefore link the signal recognition by membrane sensors with regulation of transcription via nuclear-located transcription factors that mediate plant responses to the environmental stimuli. In our study the interactions of CKI1 receiver domain with all six AHP proteins were analyzed in vivo. Using the yeast two-hybrid system we revealed that CKI1 receiver domain interacts with AHP2, AHP3 and AHP5, but not with AHP1, AHP4 and AHP6. In addition, bimolecular fluorescence complementation analysis (BiFC) was carried out in transiently transformed tobacco leaves and the interaction pattern was confirmed. Combining these two methods for analysis of protein-protein interactions in vivo we gained a rapid insight into the specificity of elements involved in a signalling cascade downstream of CKI1
dcterms:title
Interactions of the receiver domain in histidine kinase receptors with the AHP proteins Interactions of the receiver domain in histidine kinase receptors with the AHP proteins
skos:prefLabel
Interactions of the receiver domain in histidine kinase receptors with the AHP proteins Interactions of the receiver domain in histidine kinase receptors with the AHP proteins
skos:notation
RIV/00216224:14310/09:00029998!RIV10-MSM-14310___
n3:aktivita
n8:P n8:Z
n3:aktivity
P(GD204/08/H054), P(LC06034), Z(MSM0021622415)
n3:dodaniDat
n16:2010
n3:domaciTvurceVysledku
n6:6662072 n6:3807703 n6:2299186
n3:druhVysledku
n5:O
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
319927
n3:idVysledku
RIV/00216224:14310/09:00029998
n3:jazykVysledku
n10:eng
n3:klicovaSlova
two-component system; signal transduction; protein-protein interaction; BiFC; yeast two-hybrid; histidine kinase; AHP; Arabidopsis
n3:klicoveSlovo
n4:protein-protein%20interaction n4:yeast%20two-hybrid n4:signal%20transduction n4:AHP n4:histidine%20kinase n4:Arabidopsis n4:two-component%20system n4:BiFC
n3:kontrolniKodProRIV
[CD5A0D1B1853]
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n9:GD204%2F08%2FH054 n9:LC06034
n3:rokUplatneniVysledku
n16:2009
n3:tvurceVysledku
Horák, Jakub Hrdinová, Vendula Hejátko, Jan
n3:zamer
n11:MSM0021622415
n12:organizacniJednotka
14310