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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F09%3A00029652%21RIV10-MSM-14310___
rdf:type
n14:Vysledek skos:Concept
dcterms:description
The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen-seed interaction were observed with SDS-PAGE and MALDI-TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non-specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (|r|Ao0.07, 0.344), (|r|Ao0.01, 0.494), respectively, as detected by both methods. The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen-seed interaction were observed with SDS-PAGE and MALDI-TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non-specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (|r|Ao0.07, 0.344), (|r|Ao0.01, 0.494), respectively, as detected by both methods.
dcterms:title
Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening
skos:prefLabel
Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening
skos:notation
RIV/00216224:14310/09:00029652!RIV10-MSM-14310___
n3:aktivita
n9:Z n9:P
n3:aktivity
P(GA525/06/0663), P(LC06035), Z(MSM0021622415)
n3:cisloPeriodika
22
n3:dodaniDat
n16:2010
n3:domaciTvurceVysledku
n8:6955231 n8:2945541 n8:2526298 n8:9236333 n8:8696675
n3:druhVysledku
n12:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
320332
n3:idVysledku
RIV/00216224:14310/09:00029652
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Antifungal peptides; Barley; Gushing; MALDI-TOF MS; SDS-PAGE
n3:klicoveSlovo
n7:Gushing n7:SDS-PAGE n7:Antifungal%20peptides n7:MALDI-TOF%20MS n7:Barley
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[48D2B06BC549]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n19:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n17:LC06035 n17:GA525%2F06%2F0663
n3:rokUplatneniVysledku
n16:2009
n3:svazekPeriodika
32
n3:tvurceVysledku
Macuchová, Simona Preisler, Jan Farková, Marta Hégrová, Blanka Havel, Josef
n3:wos
000274217600032
n3:zamer
n6:MSM0021622415
s:issn
1615-9306
s:numberOfPages
7
n15:organizacniJednotka
14310