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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F09%3A00029571%21RIV11-GA0-14310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
We examined the composition of telomeres in chromosomes of parasitic worms, representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus tereticollis). In the Pomphorhynchus species, no hybridization signals were obtained with the nematode (TTAGGC)n, arthropod (TTAGG)n, and bdelloid (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern hybridization with P. laevis DNA. We examined the composition of telomeres in chromosomes of parasitic worms, representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus tereticollis). In the Pomphorhynchus species, no hybridization signals were obtained with the nematode (TTAGGC)n, arthropod (TTAGG)n, and bdelloid (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern hybridization with P. laevis DNA.
dcterms:title
Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization
skos:prefLabel
Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization
skos:notation
RIV/00216224:14310/09:00029571!RIV11-GA0-14310___
n3:aktivita
n18:P
n3:aktivity
P(GA524/07/1610)
n3:cisloPeriodika
11
n3:dodaniDat
n11:2011
n3:domaciTvurceVysledku
n7:9590188 n7:2983109 n7:1499327
n3:druhVysledku
n5:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
345682
n3:idVysledku
RIV/00216224:14310/09:00029571
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Acanthocephala; Platyhelminthes; chromosomes; FISH; telomere
n3:klicoveSlovo
n4:chromosomes n4:Acanthocephala n4:telomere n4:FISH n4:Platyhelminthes
n3:kodStatuVydavatele
CA - Kanada
n3:kontrolniKodProRIV
[69BF9DD3401B]
n3:nazevZdroje
Genome
n3:obor
n14:EG
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n12:GA524%2F07%2F1610
n3:rokUplatneniVysledku
n11:2009
n3:svazekPeriodika
52
n3:tvurceVysledku
Špakulová, Marta Vítková, Magda Koubková, Božena Bombarová, Marta
n3:wos
000254460100027
s:issn
1480-3321
s:numberOfPages
7
n10:organizacniJednotka
14310