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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F09%3A00029222%21RIV13-GA0-14310___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection. Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.
dcterms:title
Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
skos:prefLabel
Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
skos:notation
RIV/00216224:14310/09:00029222!RIV13-GA0-14310___
n3:aktivita
n5:P n5:Z n5:S
n3:aktivity
P(GA203/05/2256), P(GA310/09/0459), S, Z(AV0Z40500505), Z(MSM0021622415)
n3:cisloPeriodika
7
n3:dodaniDat
n4:2013
n3:domaciTvurceVysledku
n12:5051207 n12:3440656 n12:6338135 n12:6303293 n12:2412594 n12:4977459
n3:druhVysledku
n17:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
314479
n3:idVysledku
RIV/00216224:14310/09:00029222
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Magnetic microspheres; P(HEMA-co-EDMA); Staphylococcus aureus; Bacteriophage DNA; Polymerase chain reaction (PCR)
n3:klicoveSlovo
n11:P%28HEMA-co-EDMA%29 n11:Staphylococcus%20aureus n11:Bacteriophage%20DNA n11:Magnetic%20microspheres n11:Polymerase%20chain%20reaction%20%28PCR%29
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[A035347291A8]
n3:nazevZdroje
Journal of Chromatography B
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n9:GA203%2F05%2F2256 n9:GA310%2F09%2F0459
n3:rokUplatneniVysledku
n4:2009
n3:svazekPeriodika
877
n3:tvurceVysledku
Rittich, Bohuslav Pantůček, Roman Španová, Alena Doškař, Jiří Horák, Daniel Rájová, Jana
n3:wos
000264225200003
n3:zamer
n14:AV0Z40500505 n14:MSM0021622415
s:issn
1570-0232
s:numberOfPages
4
n19:organizacniJednotka
14310