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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F09%3A00029221%21RIV11-GA0-14310___
rdf:type
n17:Vysledek skos:Concept
dcterms:description
The present pilot study constitutes a proof-of-principle in the use of a quantitative LC-MS/MS based proteomic method for the comparative analysis of representative low-grade breast primary tumor tissues with and without metastases and metastasis in lymph node relative to the nonmetastatic tumor type. The study method incorporated iTRAQ stable isotope labeling, two-dimensional liquid chromatography, nanoelectrospray ionization and high resolution tandem mass spectrometry using the hybrid QqTOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were (1) to define the protein spectrum obtainable using this approach, and (2) to highlight potential candidates for verification and validation studies focused on biomarkers involved in metastatic processes in breast cancer. The study resulted in the reproducible identification of 605 nonredundant proteins. The present pilot study constitutes a proof-of-principle in the use of a quantitative LC-MS/MS based proteomic method for the comparative analysis of representative low-grade breast primary tumor tissues with and without metastases and metastasis in lymph node relative to the nonmetastatic tumor type. The study method incorporated iTRAQ stable isotope labeling, two-dimensional liquid chromatography, nanoelectrospray ionization and high resolution tandem mass spectrometry using the hybrid QqTOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were (1) to define the protein spectrum obtainable using this approach, and (2) to highlight potential candidates for verification and validation studies focused on biomarkers involved in metastatic processes in breast cancer. The study resulted in the reproducible identification of 605 nonredundant proteins.
dcterms:title
Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis
skos:prefLabel
Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis
skos:notation
RIV/00216224:14310/09:00029221!RIV11-GA0-14310___
n3:aktivita
n15:Z n15:P
n3:aktivity
P(GP203/07/P471), P(LC06035), Z(MSM0021622413), Z(MZ0MOU2005)
n3:cisloPeriodika
1
n3:dodaniDat
n12:2011
n3:domaciTvurceVysledku
n10:5713021 n10:2503786
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
305218
n3:idVysledku
RIV/00216224:14310/09:00029221
n3:jazykVysledku
n6:eng
n3:klicovaSlova
Breast cancer; biomarkers; proteomics; mass spectrometry; liquid chromatography; iTRAQ
n3:klicoveSlovo
n4:biomarkers n4:proteomics n4:iTRAQ n4:mass%20spectrometry n4:liquid%20chromatography n4:Breast%20cancer
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7DCE519989D1]
n3:nazevZdroje
Journal of Proteome Research
n3:obor
n8:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n19:GP203%2F07%2FP471 n19:LC06035
n3:rokUplatneniVysledku
n12:2009
n3:svazekPeriodika
8
n3:tvurceVysledku
Vojtěšek, Bořivoj Roumeliotis, Theodoros Hrstka, Roman Nenutil, Rudolf Garbis, Spiros D. Bouchal, Pavel
n3:wos
000262171100037
n3:zamer
n7:MSM0021622413 n7:MZ0MOU2005
s:issn
1535-3893
s:numberOfPages
12
n18:organizacniJednotka
14310