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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F09%3A00029171%21RIV10-MSM-14310___
rdf:type
n16:Vysledek skos:Concept
dcterms:description
Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops.
dcterms:title
Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
skos:prefLabel
Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
skos:notation
RIV/00216224:14310/09:00029171!RIV10-MSM-14310___
n3:aktivita
n5:P n5:Z
n3:aktivity
P(GA301/09/1917), P(GD203/09/H046), P(LC06030), Z(MSM0021622413)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2010
n3:domaciTvurceVysledku
n15:2565277
n3:druhVysledku
n18:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
352180
n3:idVysledku
RIV/00216224:14310/09:00029171
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Genome instability; recombination; DNA helicase; crossing over; Fanconi anemia
n3:klicoveSlovo
n6:Genome%20instability n6:DNA%20helicase n6:Fanconi%20anemia n6:crossing%20over n6:recombination
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[3689A13E11C5]
n3:nazevZdroje
Genes Dev.
n3:obor
n4:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
11
n3:projekt
n12:GD203%2F09%2FH046 n12:GA301%2F09%2F1917 n12:LC06030
n3:rokUplatneniVysledku
n13:2009
n3:svazekPeriodika
23
n3:tvurceVysledku
Krejčí, Lumír Dray, E. Satory, D. Ira, Grzegorz Klein, Hannah Papusha, A. Prakash, Rohit Haber, J. E. Scheller, J. Kramer, W. Sung, Patrick
n3:wos
000262369700007
n3:zamer
n19:MSM0021622413
s:issn
1549-5477
s:numberOfPages
12
n14:organizacniJednotka
14310