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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00050960%21RIV12-MSM-14310___
rdf:type
skos:Concept n19:Vysledek
rdfs:seeAlso
http://jb.asm.org/cgi/content/abstract/190/9/3155
dcterms:description
In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response. In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.
dcterms:title
aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity
skos:prefLabel
aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity
skos:notation
RIV/00216224:14310/08:00050960!RIV12-MSM-14310___
n3:aktivita
n10:Z n10:P
n3:aktivity
P(LC06030), Z(MZE0002716201)
n3:cisloPeriodika
9
n3:dodaniDat
n7:2012
n3:domaciTvurceVysledku
n15:3818780
n3:druhVysledku
n16:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n21:predkladatel
n3:idSjednocenehoVysledku
357020
n3:idVysledku
RIV/00216224:14310/08:00050960
n3:jazykVysledku
n4:eng
n3:klicovaSlova
aro mutant; Salmonella enterica; microarray; gene expression; plasmid
n3:klicoveSlovo
n5:Salmonella%20enterica n5:aro%20mutant n5:gene%20expression n5:plasmid n5:microarray
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[D4E455FE3FF6]
n3:nazevZdroje
JOURNAL OF BACTERIOLOGY
n3:obor
n18:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n13:LC06030
n3:rokUplatneniVysledku
n7:2008
n3:svazekPeriodika
190
n3:tvurceVysledku
Rychlík, Ivan Karasová, Daniela Budinská, Eva Crhánová, Magdaléna Šebková, Alena
n3:wos
000255202100008
n3:zamer
n17:MZE0002716201
s:issn
0021-9193
s:numberOfPages
6
n6:doi
10.1128/JB.00053-08
n11:organizacniJednotka
14310