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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00028599%21RIV10-AV0-14310___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
We show that double-strand breaks (DSBs) induced in DNA of human cells by gamma-radiation arise mainly in active, gene-rich, decondensed chromatin. We demonstrate that DSBs show limited movement in living cells, occasionally resulting in their permanent clustering, which poses a risk of incorrect DNA rejoining. In addition, some DSBs remain unrepaired for several days after irradiation, forming lesions repairable only with difficulty which are hazardous for genome stability. These late DSBs colocalize with heterochromatin markers (dimethylated histone H3 at lysine 9, HP1 and CENP-A proteins), despite the low density of the surrounding chromatin. This indicates that there is epigenetic silencing of loci close to unrepaired DSBs and/or stabilization of damaged decondensed chromatin loops during repair and post-repair reconstitution of chromatin structure. We show that double-strand breaks (DSBs) induced in DNA of human cells by gamma-radiation arise mainly in active, gene-rich, decondensed chromatin. We demonstrate that DSBs show limited movement in living cells, occasionally resulting in their permanent clustering, which poses a risk of incorrect DNA rejoining. In addition, some DSBs remain unrepaired for several days after irradiation, forming lesions repairable only with difficulty which are hazardous for genome stability. These late DSBs colocalize with heterochromatin markers (dimethylated histone H3 at lysine 9, HP1 and CENP-A proteins), despite the low density of the surrounding chromatin. This indicates that there is epigenetic silencing of loci close to unrepaired DSBs and/or stabilization of damaged decondensed chromatin loops during repair and post-repair reconstitution of chromatin structure.
dcterms:title
Local changes of higher-order chromatin structure during DSB-repair Local changes of higher-order chromatin structure during DSB-repair
skos:prefLabel
Local changes of higher-order chromatin structure during DSB-repair Local changes of higher-order chromatin structure during DSB-repair
skos:notation
RIV/00216224:14310/08:00028599!RIV10-AV0-14310___
n3:aktivita
n12:Z n12:P
n3:aktivity
P(1P05OC084), P(1QS500040508), P(GP204/06/P349), P(IAA1065203), P(IAA500040802), Z(AV0Z50040507), Z(AV0Z50040702)
n3:cisloPeriodika
101
n3:dodaniDat
n17:2010
n3:domaciTvurceVysledku
n9:8491313 n9:9511326 n9:5810655
n3:druhVysledku
n18:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
376999
n3:idVysledku
RIV/00216224:14310/08:00028599
n3:jazykVysledku
n19:eng
n3:klicovaSlova
DOUBLE-STRAND BREAKS; HIGH-RESOLUTION CYTOMETRY; DNA-DAMAGE; CHROMOSOME DOMAINS; ACTIVE GENES; NUCLEI; SITES; PROTEINS; CELLS; TRANSCRIPTION
n3:klicoveSlovo
n4:DNA-DAMAGE n4:SITES n4:DOUBLE-STRAND%20BREAKS n4:TRANSCRIPTION n4:HIGH-RESOLUTION%20CYTOMETRY n4:CELLS n4:ACTIVE%20GENES n4:CHROMOSOME%20DOMAINS n4:NUCLEI n4:PROTEINS
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[65C251388B55]
n3:nazevZdroje
JOURNAL OF PHYSICS CONFERENCE SERIES
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n15:IAA1065203 n15:1P05OC084 n15:GP204%2F06%2FP349 n15:IAA500040802 n15:1QS500040508
n3:rokUplatneniVysledku
n17:2008
n3:svazekPeriodika
-
n3:tvurceVysledku
Falk, Martin Gabrielová, Barbora Kozubek, Stanislav Ondřej, Vladan Lukášová, Emilie
n3:wos
BHW59
n3:zamer
n16:AV0Z50040702 n16:AV0Z50040507
s:issn
1742-6588
s:numberOfPages
18
n10:organizacniJednotka
14310