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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00028434%21RIV10-AV0-14310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer.
dcterms:title
An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes
skos:prefLabel
An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes
skos:notation
RIV/00216224:14310/08:00028434!RIV10-AV0-14310___
n3:aktivita
n15:P
n3:aktivity
P(IAA401990701)
n3:cisloPeriodika
4
n3:dodaniDat
n12:2010
n3:domaciTvurceVysledku
n4:2307049 n4:4995775 n4:3790185 n4:6132669 n4:8279314
n3:druhVysledku
n13:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
355649
n3:idVysledku
RIV/00216224:14310/08:00028434
n3:jazykVysledku
n10:eng
n3:klicovaSlova
metallothionein; voltammetry; biosensor
n3:klicoveSlovo
n5:biosensor n5:voltammetry n5:metallothionein
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[C4BA670745D6]
n3:nazevZdroje
Sensors
n3:obor
n11:CG
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n14:IAA401990701
n3:rokUplatneniVysledku
n12:2008
n3:svazekPeriodika
8
n3:tvurceVysledku
Fabrik, Ivo Baloun, Jiří Kizek, René Trnková, Libuše Adam, Vojtěch
n3:wos
000256642400013
s:issn
1424-8220
s:numberOfPages
12
n18:organizacniJednotka
14310