This HTML5 document contains 48 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n11http://localhost/temp/predkladatel/
n19http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n15http://linked.opendata.cz/resource/domain/vavai/projekt/
n14http://linked.opendata.cz/ontology/domain/vavai/
n6http://linked.opendata.cz/resource/domain/vavai/zamer/
n18http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14310%2F08%3A00026744%21RIV10-MSM-14310___/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n10http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n5http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00026744%21RIV10-MSM-14310___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Homologous recombination (HR) is a major DNA repair pathway and therefore essential for maintaining the integrity of the genome. HR is catalyzed by proteins encoded by genes of the RAD52 epistasis group, including the recombinase Rad51 and its mediator Rad52. HR proteins fused with green fluorescent protein form foci at damaged DNA reflecting the assembly of repair centers that harbor a high concentration of repair proteins. Rad52 mediates the recruitment of Rad51 and other HR proteins to DNA damage. To understand the mechanism for the assembly of Rad52-dependent DNA repair centers, we used a mutational strategy to identify a Rad52 domain essential for its recruitment to DNA repair foci. We present evidence to implicate an acidic domain in Rad52 in DNA repair focus formation. Mutations in this domain confer marked DNA damage sensitivity and recombination deficiency. Importantly, these Rad52 mutants are specifically compromised for interaction with the single-stranded DNA-binding factor RPA. Homologous recombination (HR) is a major DNA repair pathway and therefore essential for maintaining the integrity of the genome. HR is catalyzed by proteins encoded by genes of the RAD52 epistasis group, including the recombinase Rad51 and its mediator Rad52. HR proteins fused with green fluorescent protein form foci at damaged DNA reflecting the assembly of repair centers that harbor a high concentration of repair proteins. Rad52 mediates the recruitment of Rad51 and other HR proteins to DNA damage. To understand the mechanism for the assembly of Rad52-dependent DNA repair centers, we used a mutational strategy to identify a Rad52 domain essential for its recruitment to DNA repair foci. We present evidence to implicate an acidic domain in Rad52 in DNA repair focus formation. Mutations in this domain confer marked DNA damage sensitivity and recombination deficiency. Importantly, these Rad52 mutants are specifically compromised for interaction with the single-stranded DNA-binding factor RPA.
dcterms:title
Interaction with RPA Is Necessary for Rad52 Repair Center Formation and for Its Mediator Activity. Interaction with RPA Is Necessary for Rad52 Repair Center Formation and for Its Mediator Activity.
skos:prefLabel
Interaction with RPA Is Necessary for Rad52 Repair Center Formation and for Its Mediator Activity. Interaction with RPA Is Necessary for Rad52 Repair Center Formation and for Its Mediator Activity.
skos:notation
RIV/00216224:14310/08:00026744!RIV10-MSM-14310___
n3:aktivita
n4:Z n4:P
n3:aktivity
P(LC06030), P(ME 888), Z(MSM0021622413)
n3:cisloPeriodika
283
n3:dodaniDat
n5:2010
n3:domaciTvurceVysledku
n19:2565277
n3:druhVysledku
n17:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
372820
n3:idVysledku
RIV/00216224:14310/08:00026744
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Rad52; recombination mediator; DNA repair
n3:klicoveSlovo
n10:DNA%20repair n10:recombination%20mediator n10:Rad52
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[1AF309663B46]
n3:nazevZdroje
J. Biol. Chem.
n3:obor
n13:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:projekt
n15:ME%20888 n15:LC06030
n3:rokUplatneniVysledku
n5:2008
n3:svazekPeriodika
24
n3:tvurceVysledku
Shi, Idina Mortensen, Uffe Albertsen, L. Hallwyl, SC Krejčí, Lumír Plate, Iben Muller, C. Sung, Patrick
n3:wos
000260179900035
n3:zamer
n6:MSM0021622413
s:issn
0021-9258
s:numberOfPages
8
n11:organizacniJednotka
14310