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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00026700%21RIV10-MSM-14310___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationary electrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 microM, in larvae exposed to 50 ng Cd/g it was 2.0 microM and in larvae exposed to 50 microg Cd/g 2.9 microM. Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationary electrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 microM, in larvae exposed to 50 ng Cd/g it was 2.0 microM and in larvae exposed to 50 microg Cd/g 2.9 microM.
dcterms:title
A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction
skos:prefLabel
A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction
skos:notation
RIV/00216224:14310/08:00026700!RIV10-MSM-14310___
n3:aktivita
n10:Z
n3:aktivity
Z(MSM0021622412)
n3:cisloPeriodika
7
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n8:7808836 n8:4995775 n8:3790185 n8:6132669 n8:9372318 n8:2307049
n3:druhVysledku
n4:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
354156
n3:idVysledku
RIV/00216224:14310/08:00026700
n3:jazykVysledku
n17:eng
n3:klicovaSlova
electrochemistry; heavy metals; metallothionein
n3:klicoveSlovo
n15:heavy%20metals n15:electrochemistry n15:metallothionein
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[07BAB65F7B15]
n3:nazevZdroje
Sensors
n3:obor
n16:CG
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n11:2008
n3:svazekPeriodika
8
n3:tvurceVysledku
Adam, Vojtěch Hilscherová, Klára Trnková, Libuše Fabrik, Ivo Kizek, René Kostřicová, Zuzana
n3:wos
000258180500003
n3:zamer
n14:MSM0021622412
s:issn
1424-8220
s:numberOfPages
14
n13:organizacniJednotka
14310