This HTML5 document contains 75 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
n19http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14310%2F08%3A00026574%21RIV11-MSM-14310___/
dctermshttp://purl.org/dc/terms/
n13http://localhost/temp/predkladatel/
n12http://linked.opendata.cz/resource/domain/vavai/projekt/
n6http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n8http://linked.opendata.cz/ontology/domain/vavai/
n4http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n10http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n15http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00026574%21RIV11-MSM-14310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Production of soluble forms of cell surface receptors suitable for biochemical and structural studies is critical in modern membrane biology and biochemistry. We have been investigating the soluble forms of the earliest activation antigen of human leukocytes CD69. This receptor is naturally expressed at the cell surface as type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies have been a matter of controversy. We describe here the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an optimized extracellular segment corresponding to amino acids Gly70 to Lys199. This optimized protein construct has been derived from the one used previously for CD69 crystallization by its further optimalization with regard to protein stability, solubility, and easy crystallization under conditions promoting ligand binding. Production of soluble forms of cell surface receptors suitable for biochemical and structural studies is critical in modern membrane biology and biochemistry. We have been investigating the soluble forms of the earliest activation antigen of human leukocytes CD69. This receptor is naturally expressed at the cell surface as type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies have been a matter of controversy. We describe here the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an optimized extracellular segment corresponding to amino acids Gly70 to Lys199. This optimized protein construct has been derived from the one used previously for CD69 crystallization by its further optimalization with regard to protein stability, solubility, and easy crystallization under conditions promoting ligand binding.
dcterms:title
Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice
skos:prefLabel
Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice
skos:notation
RIV/00216224:14310/08:00026574!RIV11-MSM-14310___
n3:aktivita
n16:Z n16:P
n3:aktivity
P(1M0505), P(IAA500200509), P(IAA5020403), P(LC06030), P(LC07017), P(LC545), Z(AV0Z40500505), Z(AV0Z50200510), Z(AV0Z50520514), Z(MSM0021620808), Z(MSM0021620835), Z(MSM0021622413)
n3:cisloPeriodika
275
n3:dodaniDat
n15:2011
n3:domaciTvurceVysledku
n6:7750161 n6:8732124 n6:4257707 n6:7774729 n6:1901761 n6:6977685 n6:1812076 n6:3906884 n6:3984818
n3:druhVysledku
n11:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
395826
n3:idVysledku
RIV/00216224:14310/08:00026574
n3:jazykVysledku
n18:eng
n3:klicovaSlova
C-type lectin leukocyte activation plasma clearance refolding stability
n3:klicoveSlovo
n10:C-type%20lectin%20leukocyte%20activation%20plasma%20clearance%20refolding%20stability
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[FE4EAD09121F]
n3:nazevZdroje
the FEBS Journal
n3:obor
n17:CE
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
20
n3:projekt
n12:IAA500200509 n12:1M0505 n12:LC07017 n12:IAA5020403 n12:LC06030 n12:LC545
n3:rokUplatneniVysledku
n15:2008
n3:svazekPeriodika
2008
n3:tvurceVysledku
Žídek, Lukáš Vannucci, Luca Chmelík, Josef Gorčík, Lukáš Vaněk, Ondřej Brynda, Jiří Bezouška, Karel Dohnálek, Jan Borovičková, Ivana Kadeřávek, Pavel Koleno, Petr Kubíčková, Monika Pompach, Petr Kumar, Vinay Kopecký, Vladimír, Jr Novák, Petr Kavan, Daniel Sklenář, Vladimír Hofbauerová, Kateřina Hudeček, Jiří
n3:wos
000260348600011
n3:zamer
n4:AV0Z50200510 n4:AV0Z50520514 n4:MSM0021622413 n4:MSM0021620808 n4:MSM0021620835 n4:AV0Z40500505
s:issn
1742-464X
s:numberOfPages
18
n13:organizacniJednotka
14310