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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00026373%21RIV10-MSM-14310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The aim of this work was to analyse a group of 69 lactobacilli isolated from dental caries and root canals of the extracted teeth from early childhood caries (ECC) affected children up to age of six. Clinical samples obtained from individual patients were cultivated in a 5 % CO2 atmosphere on MRS agar and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization and identification of the isolates. Evaluation of obtained (GTG)5-PCR fingerprints assigned clearly 21 strains as Lactobacillus rhamnosus, 20 as Lactobacillus fermentum, 16 as Lactobacillus casei/paracasei, 7 as Lactobacillus salivarius, 4 as Lactobacillus plantarum and 1 as Lactobacillus gasseri. The aim of this work was to analyse a group of 69 lactobacilli isolated from dental caries and root canals of the extracted teeth from early childhood caries (ECC) affected children up to age of six. Clinical samples obtained from individual patients were cultivated in a 5 % CO2 atmosphere on MRS agar and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization and identification of the isolates. Evaluation of obtained (GTG)5-PCR fingerprints assigned clearly 21 strains as Lactobacillus rhamnosus, 20 as Lactobacillus fermentum, 16 as Lactobacillus casei/paracasei, 7 as Lactobacillus salivarius, 4 as Lactobacillus plantarum and 1 as Lactobacillus gasseri.
dcterms:title
Identification of lactobacilli isolated from dental caries of children Identification of lactobacilli isolated from dental caries of children
skos:prefLabel
Identification of lactobacilli isolated from dental caries of children Identification of lactobacilli isolated from dental caries of children
skos:notation
RIV/00216224:14310/08:00026373!RIV10-MSM-14310___
n3:aktivita
n5:Z n5:P
n3:aktivity
P(1M0528), Z(MSM0021622416)
n3:dodaniDat
n13:2010
n3:domaciTvurceVysledku
n12:9384839 n12:3787672 n12:1096311 n12:6666159
n3:druhVysledku
n15:O
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
371226
n3:idVysledku
RIV/00216224:14310/08:00026373
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Lactobacillus; dental caries; identification; taxonomy
n3:klicoveSlovo
n4:Lactobacillus n4:taxonomy n4:identification n4:dental%20caries
n3:kontrolniKodProRIV
[602F6F27ECDB]
n3:obor
n14:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n18:1M0528
n3:rokUplatneniVysledku
n13:2008
n3:tvurceVysledku
Kukletová, Martina Žáčková, Lenka Švec, Pavel Sedláček, Ivo
n3:zamer
n11:MSM0021622416
n10:organizacniJednotka
14310