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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F08%3A00024966%21RIV10-MSM-14310___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Gene for Burkholderia cenocepacia lectin D (BclD) was cloned in pRSET vector and expressed. However, expression was difficult, with very low yield and protein was produced mainly in insoluble form. BclD contains N-terminal domain of unknown function. As expression of lectins domains are generally high, we decided to separate N-terminal and lectin domain. Several crystals of lectin domain in different conditions were obtained using hanging drop vapour diffusion technique. These crystals are ready for data collection at ESRF (European Synchrotron Radiation Facility). Gene for Burkholderia cenocepacia lectin D (BclD) was cloned in pRSET vector and expressed. However, expression was difficult, with very low yield and protein was produced mainly in insoluble form. BclD contains N-terminal domain of unknown function. As expression of lectins domains are generally high, we decided to separate N-terminal and lectin domain. Several crystals of lectin domain in different conditions were obtained using hanging drop vapour diffusion technique. These crystals are ready for data collection at ESRF (European Synchrotron Radiation Facility).
dcterms:title
Burkholderia cenocepacia lectin D Burkholderia cenocepacia lectin D
skos:prefLabel
Burkholderia cenocepacia lectin D Burkholderia cenocepacia lectin D
skos:notation
RIV/00216224:14310/08:00024966!RIV10-MSM-14310___
n3:aktivita
n17:Z n17:P
n3:aktivity
P(GA303/06/0570), P(ME08008), Z(MSM0021622413)
n3:cisloPeriodika
3a
n3:dodaniDat
n12:2010
n3:domaciTvurceVysledku
n15:3447774 n15:3543331 n15:5522064
n3:druhVysledku
n19:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
358650
n3:idVysledku
RIV/00216224:14310/08:00024966
n3:jazykVysledku
n7:eng
n3:klicovaSlova
Burkholderia cenocepacia; lectin; structure
n3:klicoveSlovo
n9:Burkholderia%20cenocepacia n9:structure n9:lectin
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[E2EC73703E9A]
n3:nazevZdroje
Materials Structure in Chemistry, Biology, Physics and Technology
n3:obor
n6:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n8:GA303%2F06%2F0570 n8:ME08008
n3:rokUplatneniVysledku
n12:2008
n3:svazekPeriodika
15
n3:tvurceVysledku
Wimmerová, Michaela Malinovská, Lenka Pokorná, Marie
n3:zamer
n16:MSM0021622413
s:issn
1211-5894
s:numberOfPages
2
n11:organizacniJednotka
14310