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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00050868%21RIV12-MSM-14310___
rdf:type
n16:Vysledek skos:Concept
dcterms:description
The main aim of this work was to prepare and determine apoMT by adsorptive transfer stripping technique (AdTS) in connection with differential pulse voltammetry (DPV). Particularly, we investigated the electrochemical behavior of MT measured on the surface of hanging mercury drop electrode in the presence of sodium chloride as supporting electrolyte using AdTS DPV with respect to study the effects of MT signals by different concentrations and pH's of the electrolyte. Then, we aimed at utilizing this technique to observe changes in MTwhich are dependent on using strong chelating compounds ethylendiamine-N, N, N', N'-tetraacetic acid (EDTA). Thanks to ability of EDTA to bind heavy metals from active center of enzymes and regulation proteins, we were able to prepare apoMT; that means, MT without any metal ion bound in its structure. The main aim of this work was to prepare and determine apoMT by adsorptive transfer stripping technique (AdTS) in connection with differential pulse voltammetry (DPV). Particularly, we investigated the electrochemical behavior of MT measured on the surface of hanging mercury drop electrode in the presence of sodium chloride as supporting electrolyte using AdTS DPV with respect to study the effects of MT signals by different concentrations and pH's of the electrolyte. Then, we aimed at utilizing this technique to observe changes in MTwhich are dependent on using strong chelating compounds ethylendiamine-N, N, N', N'-tetraacetic acid (EDTA). Thanks to ability of EDTA to bind heavy metals from active center of enzymes and regulation proteins, we were able to prepare apoMT; that means, MT without any metal ion bound in its structure.
dcterms:title
Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry
skos:prefLabel
Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry
skos:notation
RIV/00216224:14310/07:00050868!RIV12-MSM-14310___
n3:aktivita
n7:P n7:Z
n3:aktivity
P(LC06035), Z(MSM0021622412)
n3:cisloPeriodika
2-3
n3:dodaniDat
n4:2012
n3:domaciTvurceVysledku
n14:2104237 n14:3790185 n14:2307049 n14:8740658
n3:druhVysledku
n9:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
416632
n3:idVysledku
RIV/00216224:14310/07:00050868
n3:jazykVysledku
n20:eng
n3:klicovaSlova
metallothionein; metaloproteins; voltammetry
n3:klicoveSlovo
n15:voltammetry n15:metallothionein n15:metaloproteins
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[7770B79E4BAD]
n3:nazevZdroje
Electroanalysis
n3:obor
n13:CG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n18:LC06035
n3:rokUplatneniVysledku
n4:2007
n3:svazekPeriodika
19
n3:tvurceVysledku
Adam, Vojtěch Zítka, Ondřej Kizek, René Křížková, Soňa Trnková, Libuše
n3:zamer
n11:MSM0021622412
s:issn
1040-0397
s:numberOfPages
9
n19:doi
10.1002/elan.200603738
n10:organizacniJednotka
14310