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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00023007%21RIV08-MSM-14310___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, API Zym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruently separated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in several strains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR fingerprinting. The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, API Zym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruently separated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in several strains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR fingerprinting. Charakterizace kmenů Staphylococcus sp. pomocí metody ribotypizace, analýzy celkových buněčných proteinů a (GTG)5-PCR.
dcterms:title
Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms Charakterizace kmenů Staphylococcus sp. deponovaných v České sbírce mikroorganismů
skos:prefLabel
Charakterizace kmenů Staphylococcus sp. deponovaných v České sbírce mikroorganismů Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms
skos:notation
RIV/00216224:14310/07:00023007!RIV08-MSM-14310___
n3:strany
218-219
n3:aktivita
n19:Z
n3:aktivity
Z(MSM0021622416)
n3:dodaniDat
n14:2008
n3:domaciTvurceVysledku
n9:8037051 n9:9384839 n9:3787672
n3:druhVysledku
n4:D
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
413356
n3:idVysledku
RIV/00216224:14310/07:00023007
n3:jazykVysledku
n7:eng
n3:klicovaSlova
Staphylococcus sp.; ribotyping; whole-cell protein analysis; rep-PCR
n3:klicoveSlovo
n8:ribotyping n8:rep-PCR n8:Staphylococcus%20sp. n8:whole-cell%20protein%20analysis
n3:kontrolniKodProRIV
[C4747FCE01B5]
n3:mistoKonaniAkce
Goslar, Germany
n3:mistoVydani
Braunschweig
n3:nazevZdroje
Proceedings of the 11th International Conference on Culture Collections
n3:obor
n21:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n14:2007
n3:tvurceVysledku
Sedláček, Ivo Švec, Pavel Nováková, Dana
n3:typAkce
n20:WRD
n3:zahajeniAkce
2007-10-07+02:00
n3:zamer
n15:MSM0021622416
s:numberOfPages
2
n5:hasPublisher
DSMZ- German Collection of Microorganisms and Cell Cultures
n13:isbn
978-3-00-022417-1
n17:organizacniJednotka
14310