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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00022879%21RIV10-MSM-14310___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections. Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.
dcterms:title
Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor
skos:prefLabel
Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor
skos:notation
RIV/00216224:14310/07:00022879!RIV10-MSM-14310___
n3:aktivita
n13:Z n13:P
n3:aktivity
P(OSVTUO2006003), Z(MSM0021622413)
n3:cisloPeriodika
23
n3:dodaniDat
n8:2010
n3:domaciTvurceVysledku
n10:3876284 n10:6669719
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
449526
n3:idVysledku
RIV/00216224:14310/07:00022879
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Amperometric biosensor; Screen-printed sensor; Immunoassay; Francisella tularensis
n3:klicoveSlovo
n4:Screen-printed%20sensor n4:Immunoassay n4:Francisella%20tularensis n4:Amperometric%20biosensor
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[0DCD40B9ED3A]
n3:nazevZdroje
Electroanalysis
n3:obor
n5:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n15:OSVTUO2006003
n3:rokUplatneniVysledku
n8:2007
n3:svazekPeriodika
19
n3:tvurceVysledku
Skládal, Petr Pohanka, Miroslav
n3:zamer
n11:MSM0021622413
s:issn
1040-0397
s:numberOfPages
6
n18:organizacniJednotka
14310