This HTML5 document contains 45 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n12http://localhost/temp/predkladatel/
n15http://linked.opendata.cz/resource/domain/vavai/projekt/
n9http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n13http://linked.opendata.cz/ontology/domain/vavai/
n16http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n5http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n17http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14310%2F07%3A00021740%21RIV10-MSM-14310___/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n19http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n7http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00021740%21RIV10-MSM-14310___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2. A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2.
dcterms:title
Diagnosis of tularemia using piezoelectric biosensor technology Diagnosis of tularemia using piezoelectric biosensor technology
skos:prefLabel
Diagnosis of tularemia using piezoelectric biosensor technology Diagnosis of tularemia using piezoelectric biosensor technology
skos:notation
RIV/00216224:14310/07:00021740!RIV10-MSM-14310___
n3:aktivita
n8:P n8:Z
n3:aktivity
P(OBVTUO2003001), Z(MSM0021622413)
n3:cisloPeriodika
1
n3:dodaniDat
n7:2010
n3:domaciTvurceVysledku
n9:3876284 n9:6669719
n3:druhVysledku
n11:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
416951
n3:idVysledku
RIV/00216224:14310/07:00021740
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Piezoelectric biosensor; Francisella tularensis; Bioterrorism; Tularemia; Rapid detection; Immunosensor
n3:klicoveSlovo
n5:Bioterrorism n5:Piezoelectric%20biosensor n5:Francisella%20tularensis n5:Immunosensor n5:Tularemia n5:Rapid%20detection
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[BE4702056406]
n3:nazevZdroje
Talanta
n3:obor
n14:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n15:OBVTUO2003001
n3:rokUplatneniVysledku
n7:2007
n3:svazekPeriodika
71
n3:tvurceVysledku
Pohanka, Miroslav Skládal, Petr Pavliš, Oto
n3:zamer
n16:MSM0021622413
s:issn
0039-9140
s:numberOfPages
5
n12:organizacniJednotka
14310