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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00020478%21RIV10-GA0-14310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Magnetite nanoparticles ca. 14 nm in diameter were obtained by chemical coprecipitation of Fe(II) and Fe(III) salts with ammonia in the presence of poly(ethylene glycol) (PEG). Magnetic poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization in poly(vinylpyrrolidone) (PVP)-stabilized aqueous ethanol with PEG-coated magnetite nanoparticles. Magnetic PGMA microspheres were hydrolyzed and carboxyl groups introduced by oxidation with KMnO4. Such a carrier was used for isolation of DNA. The particles reversibly bound DNA in the presence of high concentration of PEG 6,000 and sodium chloride. Bacterial DNA was isolated from crude cell lysates of different dairy products (butter milk, cheese, yoghurt), probiotic tablets or from cell lyophilisates. The presence of Bifidobacterium and Lactobacillus DNA in samples was identified by PCR amplification. Magnetite nanoparticles ca. 14 nm in diameter were obtained by chemical coprecipitation of Fe(II) and Fe(III) salts with ammonia in the presence of poly(ethylene glycol) (PEG). Magnetic poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization in poly(vinylpyrrolidone) (PVP)-stabilized aqueous ethanol with PEG-coated magnetite nanoparticles. Magnetic PGMA microspheres were hydrolyzed and carboxyl groups introduced by oxidation with KMnO4. Such a carrier was used for isolation of DNA. The particles reversibly bound DNA in the presence of high concentration of PEG 6,000 and sodium chloride. Bacterial DNA was isolated from crude cell lysates of different dairy products (butter milk, cheese, yoghurt), probiotic tablets or from cell lyophilisates. The presence of Bifidobacterium and Lactobacillus DNA in samples was identified by PCR amplification.
dcterms:title
Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products
skos:prefLabel
Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products
skos:notation
RIV/00216224:14310/07:00020478!RIV10-GA0-14310___
n4:aktivita
n10:P n10:Z
n4:aktivity
P(GA525/05/0311), Z(AV0Z40500505)
n4:cisloPeriodika
1
n4:dodaniDat
n7:2010
n4:domaciTvurceVysledku
n15:2412594 n15:4977459
n4:druhVysledku
n6:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
412642
n4:idVysledku
RIV/00216224:14310/07:00020478
n4:jazykVysledku
n19:eng
n4:klicovaSlova
magnetic particles; DNA; magnetite; PCR; dispersion polymerization; bacterial DNA assay
n4:klicoveSlovo
n5:DNA n5:PCR n5:bacterial%20DNA%20assay n5:magnetite n5:magnetic%20particles n5:dispersion%20polymerization
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[6D8192BC0512]
n4:nazevZdroje
Journal of Magnetism and Magnetic Materials
n4:obor
n13:CD
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
3
n4:projekt
n14:GA525%2F05%2F0311
n4:rokUplatneniVysledku
n7:2007
n4:svazekPeriodika
311
n4:tvurceVysledku
Španová, Alena Horák, Daniel Rittich, Bohuslav
n4:zamer
n11:AV0Z40500505
s:issn
0304-8853
s:numberOfPages
6
n16:organizacniJednotka
14310