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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00020260%21RIV11-GA0-14310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Thirteen polymorphic microsatellites were isolated from Leuciscus cephalus, a widespread cyprinid species with great ecological tolerance. Together with the cross-species amplification of six additional loci originally published for three cyprinid fish species, we optimized a multiplex panel for L. cephalus allowing the genotyping of 19 polymorphic loci. Number of alleles and heterozygosity per locus in a sample of 20 fish individuals ranged from two to sixteen and from 0.05 to 0.90, respectively. These primers will be useful in determining the population structure of L. cephalus. In addition, successful cross-amplification was obtained for several species of Cyprinidae. Thirteen polymorphic microsatellites were isolated from Leuciscus cephalus, a widespread cyprinid species with great ecological tolerance. Together with the cross-species amplification of six additional loci originally published for three cyprinid fish species, we optimized a multiplex panel for L. cephalus allowing the genotyping of 19 polymorphic loci. Number of alleles and heterozygosity per locus in a sample of 20 fish individuals ranged from two to sixteen and from 0.05 to 0.90, respectively. These primers will be useful in determining the population structure of L. cephalus. In addition, successful cross-amplification was obtained for several species of Cyprinidae.
dcterms:title
Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae. Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae.
skos:prefLabel
Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae. Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae.
skos:notation
RIV/00216224:14310/07:00020260!RIV11-GA0-14310___
n3:aktivita
n10:Z n10:P
n3:aktivity
P(GA524/04/1128), P(LC522), Z(AV0Z60930519), Z(MSM0021622416)
n3:cisloPeriodika
6
n3:dodaniDat
n18:2011
n3:domaciTvurceVysledku
n19:7166680 n19:3741613
n3:druhVysledku
n12:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
427631
n3:idVysledku
RIV/00216224:14310/07:00020260
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Leuciscus; Cyprinidae; microsatellites; primers; cross-amplification
n3:klicoveSlovo
n4:Cyprinidae n4:Leuciscus n4:microsatellites n4:cross-amplification n4:primers
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[E161497B5E2C]
n3:nazevZdroje
Molecular Ecology Notes
n3:obor
n13:EG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n8:LC522 n8:GA524%2F04%2F1128
n3:rokUplatneniVysledku
n18:2007
n3:svazekPeriodika
7
n3:tvurceVysledku
Martin, Jean-François Vyskočilová, Martina Vetešníková Šimková, Andrea
n3:wos
000250647700065
n3:zamer
n7:AV0Z60930519 n7:MSM0021622416
s:issn
1471-8278
s:numberOfPages
5
n17:organizacniJednotka
14310