This HTML5 document contains 43 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n15http://localhost/temp/predkladatel/
n16http://linked.opendata.cz/resource/domain/vavai/projekt/
n14http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n17http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14310%2F07%3A00020172%21RIV10-MSM-14310___/
n9http://linked.opendata.cz/ontology/domain/vavai/
n4http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n6http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n12http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n11http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00020172%21RIV10-MSM-14310___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
The possibility of integration of the short end injection mode in the EMMA methodology is demonstrated in this work on the kinetic studies of haloalkane dehalogenase and rhodanese enzymatic reactions. The essential validations of the EMMA methods combined with the short end and long end injection modes were performed first to confirm their accuracy. The qualitative and quantitative parameters of both approaches such as repetabilities of migration times and peak areas, limits of detection and correlation coefficients were in acceptable ranges. In addition estimated Michaelis constants for the corresponding substrate(s) were comparable being in accordance with previous literature data. Moreover the ping-pong reaction mechanism of rhodanese reaction was confirmed by means of both injection modes. This combination thus preserves the benefits of these instrumental approaches. Whereas the short-end injection procedure brought 5 6.5 times reduction of the analysis time and 2. The possibility of integration of the short end injection mode in the EMMA methodology is demonstrated in this work on the kinetic studies of haloalkane dehalogenase and rhodanese enzymatic reactions. The essential validations of the EMMA methods combined with the short end and long end injection modes were performed first to confirm their accuracy. The qualitative and quantitative parameters of both approaches such as repetabilities of migration times and peak areas, limits of detection and correlation coefficients were in acceptable ranges. In addition estimated Michaelis constants for the corresponding substrate(s) were comparable being in accordance with previous literature data. Moreover the ping-pong reaction mechanism of rhodanese reaction was confirmed by means of both injection modes. This combination thus preserves the benefits of these instrumental approaches. Whereas the short-end injection procedure brought 5 6.5 times reduction of the analysis time and 2.
dcterms:title
Integration of short-end injection procedure into EMMA methodology. Integration of short-end injection procedure into EMMA methodology.
skos:prefLabel
Integration of short-end injection procedure into EMMA methodology. Integration of short-end injection procedure into EMMA methodology.
skos:notation
RIV/00216224:14310/07:00020172!RIV10-MSM-14310___
n3:aktivita
n8:Z n8:P
n3:aktivity
P(GA203/06/0047), P(LC06023), Z(MSM0021622413)
n3:cisloPeriodika
1-2
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n14:3195201 n14:5235979
n3:druhVysledku
n18:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
426955
n3:idVysledku
RIV/00216224:14310/07:00020172
n3:jazykVysledku
n12:eng
n3:klicovaSlova
CE; EMMA substrate; inhibition; haloalkane dehalogenase
n3:klicoveSlovo
n6:CE n6:haloalkane%20dehalogenase n6:inhibition n6:EMMA%20substrate
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[0891C6A97929]
n3:nazevZdroje
Journal of Chromatography A
n3:obor
n19:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n16:GA203%2F06%2F0047 n16:LC06023
n3:rokUplatneniVysledku
n11:2007
n3:svazekPeriodika
1155
n3:tvurceVysledku
Němec, Tomáš Glatz, Zdeněk
n3:zamer
n4:MSM0021622413
s:issn
0021-9673
s:numberOfPages
5
n15:organizacniJednotka
14310