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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F07%3A00020038%21RIV10-MSM-14310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Substrate inhibition is a common phenomenon in enzyme kinetics. We report here for the first time its study by a combination of the electrophoretically mediated microanalysis (EMMA) methodology with a partial filling technique. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. In the case of haloalkane dehalogenase, a model enzyme selected for this study, the enzymatic reaction was performed in 20 mM glycine buffer (pH 8.6) whereas 20 mM beta-alanine - hydrochloric acid buffer (pH 3.5) was used as a background electrolyte in combination with direct detection at 200 nm. The whole study was performed on poorly soluble brominated substrate - 1,2-dibromoethane. Substrate inhibition is a common phenomenon in enzyme kinetics. We report here for the first time its study by a combination of the electrophoretically mediated microanalysis (EMMA) methodology with a partial filling technique. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. In the case of haloalkane dehalogenase, a model enzyme selected for this study, the enzymatic reaction was performed in 20 mM glycine buffer (pH 8.6) whereas 20 mM beta-alanine - hydrochloric acid buffer (pH 3.5) was used as a background electrolyte in combination with direct detection at 200 nm. The whole study was performed on poorly soluble brominated substrate - 1,2-dibromoethane.
dcterms:title
Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary.
skos:prefLabel
Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary. Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary.
skos:notation
RIV/00216224:14310/07:00020038!RIV10-MSM-14310___
n3:aktivita
n7:P n7:Z
n3:aktivity
P(GA203/06/0047), P(LC06023), Z(MSM0021622413)
n3:cisloPeriodika
1-2
n3:dodaniDat
n4:2010
n3:domaciTvurceVysledku
n9:5235979 n9:1429019 n9:7612656 n9:3195201
n3:druhVysledku
n19:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
453316
n3:idVysledku
RIV/00216224:14310/07:00020038
n3:jazykVysledku
n18:eng
n3:klicovaSlova
CE; EMMA substrate; inhibition; haloalkane dehalogenase
n3:klicoveSlovo
n5:CE n5:haloalkane%20dehalogenase n5:EMMA%20substrate n5:inhibition
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[B515974CE304]
n3:nazevZdroje
Journal of Chromatography A
n3:obor
n14:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n16:LC06023 n16:GA203%2F06%2F0047
n3:rokUplatneniVysledku
n4:2007
n3:svazekPeriodika
1150
n3:tvurceVysledku
Chaloupková, Radka Němec, Tomáš Papežová, Kateřina Glatz, Zdeněk
n3:zamer
n12:MSM0021622413
s:issn
0021-9673
s:numberOfPages
5
n6:organizacniJednotka
14310