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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F06%3A00018084%21RIV10-MSM-14310___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days. Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days.
dcterms:title
In situ hybridization for mRNA detection in Arabidopsis tissue sections. In situ hybridization for mRNA detection in Arabidopsis tissue sections.
skos:prefLabel
In situ hybridization for mRNA detection in Arabidopsis tissue sections. In situ hybridization for mRNA detection in Arabidopsis tissue sections.
skos:notation
RIV/00216224:14310/06:00018084!RIV10-MSM-14310___
n4:aktivita
n7:P n7:Z
n4:aktivity
P(LC06034), Z(MSM0021622415)
n4:cisloPeriodika
3
n4:dodaniDat
n8:2010
n4:domaciTvurceVysledku
n16:3730832 n16:6662072
n4:druhVysledku
n18:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
479124
n4:idVysledku
RIV/00216224:14310/06:00018084
n4:jazykVysledku
n13:eng
n4:klicovaSlova
in situ mRNA localization; tissue sections; plants
n4:klicoveSlovo
n5:in%20situ%20mRNA%20localization n5:plants n5:tissue%20sections
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[8B1EB0AE015A]
n4:nazevZdroje
Nature Protocols
n4:obor
n15:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
5
n4:projekt
n14:LC06034
n4:rokUplatneniVysledku
n8:2006
n4:svazekPeriodika
1
n4:tvurceVysledku
Friml, Jiří Brewer, P. Benková, Eva Hejátko, Jan Heisler, M.
n4:zamer
n17:MSM0021622415
s:issn
1745-2473
s:numberOfPages
6
n19:organizacniJednotka
14310