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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F06%3A00017201%21RIV10-MSM-14310___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
A typical MS-assisted proteomic routine involves protease trypsin to generate specific fragments for further analysis. As the enzyme shows marginal termostability and undegoes intense autolysis, it became important to improve its properties in this regard. Conjugation of trypsin with e.g. oligosaccharides (maltotriose, raffinose, stachyose) is one the possible ways to solve these problems. Another way might be use of proteases of similar specifity from bacteria. Both approches were tested and particular proteases were characterised by means of their biochemical (pI, mol.mass, kinetics) and MS-related properties (cleavage efficiency). A typical MS-assisted proteomic routine involves protease trypsin to generate specific fragments for further analysis. As the enzyme shows marginal termostability and undegoes intense autolysis, it became important to improve its properties in this regard. Conjugation of trypsin with e.g. oligosaccharides (maltotriose, raffinose, stachyose) is one the possible ways to solve these problems. Another way might be use of proteases of similar specifity from bacteria. Both approches were tested and particular proteases were characterised by means of their biochemical (pI, mol.mass, kinetics) and MS-related properties (cleavage efficiency).
dcterms:title
Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process
skos:prefLabel
Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process
skos:notation
RIV/00216224:14310/06:00017201!RIV10-MSM-14310___
n4:aktivita
n17:Z
n4:aktivity
Z(MSM0021622413)
n4:dodaniDat
n15:2010
n4:domaciTvurceVysledku
n11:9708790 n11:1892363
n4:druhVysledku
n5:O
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
486519
n4:idVysledku
RIV/00216224:14310/06:00017201
n4:jazykVysledku
n10:eng
n4:klicovaSlova
trypsin; tryptic digestion; PMF; modified trypsin
n4:klicoveSlovo
n12:trypsin n12:tryptic%20digestion n12:PMF n12:modified%20trypsin
n4:kontrolniKodProRIV
[8BBA2D53198B]
n4:obor
n7:CE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:rokUplatneniVysledku
n15:2006
n4:tvurceVysledku
Šebela, Marek Havliš, Jan Wielsch, Natalie Shevchenko, Andrej Štosová, Tatiana Tarkowski, Petr Thomas, Henrik
n4:zamer
n14:MSM0021622413
n8:organizacniJednotka
14310