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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F06%3A00015836%21RIV10-MSM-14310___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Bacteriophage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. The genomes of polyvalent staphylococcal phages exceed 125 kbp and include about 200 putative open reading frames. Our study was focused on characterization of the phage proteome, especially on identification of phage virion proteins. Combination of both one- and two-dimensional electrophoreses (1-DE and 2-DE) followed by peptide mass fingerprinting using MALDI-TOF mass spectrometry, led to the identification of 24 virion proteins of the phage 812. Twenty of them were not identified by proteome analysis of closely related staphylococcal phages K and G1 yet. Although the phage proteome is much simpler compared to that of cellular organisms, using 2-DE in addition to 1-DE significantly increased the number of identified proteins. Bacteriophage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. The genomes of polyvalent staphylococcal phages exceed 125 kbp and include about 200 putative open reading frames. Our study was focused on characterization of the phage proteome, especially on identification of phage virion proteins. Combination of both one- and two-dimensional electrophoreses (1-DE and 2-DE) followed by peptide mass fingerprinting using MALDI-TOF mass spectrometry, led to the identification of 24 virion proteins of the phage 812. Twenty of them were not identified by proteome analysis of closely related staphylococcal phages K and G1 yet. Although the phage proteome is much simpler compared to that of cellular organisms, using 2-DE in addition to 1-DE significantly increased the number of identified proteins.
dcterms:title
Proteomic characterization of the polyvalent staphylophage 812 Proteomic characterization of the polyvalent staphylophage 812
skos:prefLabel
Proteomic characterization of the polyvalent staphylophage 812 Proteomic characterization of the polyvalent staphylophage 812
skos:notation
RIV/00216224:14310/06:00015836!RIV10-MSM-14310___
n3:aktivita
n4:Z n4:P
n3:aktivity
P(GA203/03/0515), Z(MSM0021622415)
n3:dodaniDat
n9:2010
n3:domaciTvurceVysledku
n11:3764915 n11:9725741 n11:9236333 n11:4186672 n11:3431525 n11:4568567 n11:3440656 n11:5051207
n3:druhVysledku
n16:O
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
495704
n3:idVysledku
RIV/00216224:14310/06:00015836
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Staphylococcus aureus; medical microbiology; molecular diagnostics; phage therapy; proteomics
n3:klicoveSlovo
n14:medical%20microbiology n14:phage%20therapy n14:proteomics n14:molecular%20diagnostics n14:Staphylococcus%20aureus
n3:kontrolniKodProRIV
[A7D85661D03C]
n3:obor
n7:EB
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
8
n3:projekt
n13:GA203%2F03%2F0515
n3:rokUplatneniVysledku
n9:2006
n3:tvurceVysledku
Hernychová, Lenka Doškař, Jiří Preisler, Jan Zdráhal, Zbyněk Konečná, Hana Eyer, Luděk Růžičková, Vladislava Pantůček, Roman
n3:zamer
n8:MSM0021622415
n15:organizacniJednotka
14310