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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F06%3A00015758%21RIV10-MSM-14310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Number of authors have concerned with electrochemical analysis of metallothionein. Recently new electroanalytical techniques enabling determination of MT at picomole level has been suggested. The aim of the presented work was to show advantages and disadvantages of the different electrochemical procedures, which are commonly used for the detection of MT(i) cyclic voltammetry, (ii) differential pulse voltammetry, and (iii) Brdicka reaction. Primarily we aimed on improvement of the mentioned techniques. Using of reducing agent (tris(2-carboxyethyl)phosphine) and combination of the mentioned method with adsorptive transfer stripping technique (AdTS) were the main improvements of the voltammetric method. The detection limits of metallothionein measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and DPV Brdicka reaction were 0.5 pmol, 4 fmol and 10 amol, respectively. Number of authors have concerned with electrochemical analysis of metallothionein. Recently new electroanalytical techniques enabling determination of MT at picomole level has been suggested. The aim of the presented work was to show advantages and disadvantages of the different electrochemical procedures, which are commonly used for the detection of MT(i) cyclic voltammetry, (ii) differential pulse voltammetry, and (iii) Brdicka reaction. Primarily we aimed on improvement of the mentioned techniques. Using of reducing agent (tris(2-carboxyethyl)phosphine) and combination of the mentioned method with adsorptive transfer stripping technique (AdTS) were the main improvements of the voltammetric method. The detection limits of metallothionein measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and DPV Brdicka reaction were 0.5 pmol, 4 fmol and 10 amol, respectively.
dcterms:title
Attomole voltammetric determination of metallothionein Attomole voltammetric determination of metallothionein
skos:prefLabel
Attomole voltammetric determination of metallothionein Attomole voltammetric determination of metallothionein
skos:notation
RIV/00216224:14310/06:00015758!RIV10-MSM-14310___
n3:aktivita
n11:V n11:Z n11:P
n3:aktivity
P(GP525/04/P132), V, Z(AV0Z50040507), Z(MSM0021622412)
n3:cisloPeriodika
24
n3:dodaniDat
n13:2010
n3:domaciTvurceVysledku
n10:4995775 n10:3363104 n10:4637844 n10:2307049 n10:6563902 n10:3790185
n3:druhVysledku
n18:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
466309
n3:idVysledku
RIV/00216224:14310/06:00015758
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Metallothionein; Mercury electrode; Differential pulse voltammetry; Cyclic voltammetry; Brdicka reaction; Adsorptive transfer stripping technique; Reducing agent; Tris(2-carboxyethyl)phosphine; Lead poisoning; Human blood serum
n3:klicoveSlovo
n7:Differential%20pulse%20voltammetry n7:Tris%282-carboxyethyl%29phosphine n7:Cyclic%20voltammetry n7:Lead%20poisoning n7:Adsorptive%20transfer%20stripping%20technique n7:Brdicka%20reaction n7:Metallothionein n7:Human%20blood%20serum n7:Mercury%20electrode n7:Reducing%20agent
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[A89A1831BA46]
n3:nazevZdroje
Electrochimica Acta
n3:obor
n16:CG
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
10
n3:projekt
n9:GP525%2F04%2FP132
n3:rokUplatneniVysledku
n13:2006
n3:svazekPeriodika
51
n3:tvurceVysledku
Potěšil, David Mikelová, Radka Jelen, František Kukačka, Jiří Petrlová, Jitka Trnková, Libuše Kizek, René Blaštík, Ondřej Průša, Richard Adam, Vojtěch
n3:zamer
n4:MSM0021622412 n4:AV0Z50040507
s:issn
0013-4686
s:numberOfPages
8
n19:organizacniJednotka
14310