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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F06%3A00015742%21RIV10-MSM-14310___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
We explored the identity and function of an unknown protein that was greatly overexpressed in v-myb-transformed BM2 monoblasts undergoing differentiation to macrophage-like cells. We identified this protein as vimentin, the intermediate filament protein. We show that an increased level of the vimentin protein results from activation of the vimentin gene promoter occurring in monoblastic cells induced to differentiate by multiple agents. Furthermore, our studies reveal that the vimentin gene promoter is stimulated by Myb and Jun proteins, the key transcriptional regulators of myeloid maturation. Silencing of vimentin gene expression using siRNA markedly suppressed the ability of BM2 cells to form macrophage polykaryons active in phagocytosis and producing reactive oxygen species. These findings document that up-regulation of vimentin gene expression is important for formation of fully active macrophage-like cells. We explored the identity and function of an unknown protein that was greatly overexpressed in v-myb-transformed BM2 monoblasts undergoing differentiation to macrophage-like cells. We identified this protein as vimentin, the intermediate filament protein. We show that an increased level of the vimentin protein results from activation of the vimentin gene promoter occurring in monoblastic cells induced to differentiate by multiple agents. Furthermore, our studies reveal that the vimentin gene promoter is stimulated by Myb and Jun proteins, the key transcriptional regulators of myeloid maturation. Silencing of vimentin gene expression using siRNA markedly suppressed the ability of BM2 cells to form macrophage polykaryons active in phagocytosis and producing reactive oxygen species. These findings document that up-regulation of vimentin gene expression is important for formation of fully active macrophage-like cells.
dcterms:title
Role of vimentin in regulation of monocyte/macrophage differentiation Role of vimentin in regulation of monocyte/macrophage differentiation
skos:prefLabel
Role of vimentin in regulation of monocyte/macrophage differentiation Role of vimentin in regulation of monocyte/macrophage differentiation
skos:notation
RIV/00216224:14310/06:00015742!RIV10-MSM-14310___
n4:aktivita
n12:Z n12:P
n4:aktivity
P(GA301/03/1055), P(GP301/03/D022), Z(MSM0021622415)
n4:cisloPeriodika
6
n4:dodaniDat
n14:2010
n4:domaciTvurceVysledku
n16:5687683 n16:8996288 n16:4568567 n16:6659446 n16:6234348 n16:9725741 n16:8780757
n4:druhVysledku
n17:J
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
497922
n4:idVysledku
RIV/00216224:14310/06:00015742
n4:jazykVysledku
n18:eng
n4:klicovaSlova
vimentin; differentiation; macrophage; phagocytosis; proliferation
n4:klicoveSlovo
n8:vimentin n8:differentiation n8:proliferation n8:macrophage n8:phagocytosis
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[3ED08B0B36FC]
n4:nazevZdroje
Differentiation
n4:obor
n5:EB
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
7
n4:projekt
n11:GA301%2F03%2F1055 n11:GP301%2F03%2FD022
n4:rokUplatneniVysledku
n14:2006
n4:svazekPeriodika
74
n4:tvurceVysledku
Pelková, Vendula Ondroušková, Eva Šmarda, Jan Beneš, Petr Konečná, Hana Mužík, Jan Zdráhal, Zbyněk
n4:zamer
n13:MSM0021622415
s:issn
0301-4681
s:numberOfPages
12
n19:organizacniJednotka
14310