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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F06%3A00015727%21RIV10-MSM-14310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
A comparative analysis of the protein composition of Acidithiobacillus ferrooxidans cells grown on elemental sulfur and ferrous iron was performed. A newly developed protocol involving immobilized pH gradients, improved protein reduction, mass spectrometry protein identification and full genome sequence information was applied. This approach resulted in more than 1300 protein spots displayed in broad and basic pH ranges, the best At. ferrooxidans proteome resolution to date. A comparative image analysis revealed that the proteome was significantly influenced by the growth type, and allowed for the detection of many physiologically important proteins. Among them were sulfate adenylyltransferase and sulfide dehydrogenase, which are involved in sulfate assimilation and sulfide metabolism, respectively. Many other proteins were related to important processes like cell attachment and electron transport. Co-migration of phosphate and sulfate transport proteins was also observed. A comparative analysis of the protein composition of Acidithiobacillus ferrooxidans cells grown on elemental sulfur and ferrous iron was performed. A newly developed protocol involving immobilized pH gradients, improved protein reduction, mass spectrometry protein identification and full genome sequence information was applied. This approach resulted in more than 1300 protein spots displayed in broad and basic pH ranges, the best At. ferrooxidans proteome resolution to date. A comparative image analysis revealed that the proteome was significantly influenced by the growth type, and allowed for the detection of many physiologically important proteins. Among them were sulfate adenylyltransferase and sulfide dehydrogenase, which are involved in sulfate assimilation and sulfide metabolism, respectively. Many other proteins were related to important processes like cell attachment and electron transport. Co-migration of phosphate and sulfate transport proteins was also observed.
dcterms:title
Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry
skos:prefLabel
Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry
skos:notation
RIV/00216224:14310/06:00015727!RIV10-MSM-14310___
n3:aktivita
n9:Z n9:P
n3:aktivity
P(GA525/04/1309), Z(MSM0021622413), Z(MSM0021622415)
n3:cisloPeriodika
15
n3:dodaniDat
n16:2010
n3:domaciTvurceVysledku
n6:6890091 n6:2503786 n6:8541965 n6:3331091 n6:4568567
n3:druhVysledku
n11:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
495703
n3:idVysledku
RIV/00216224:14310/06:00015727
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Acidithiobacilus ferrooxidans; Iron oxidation; Protein reduction; Proteome; Sulfur oxidation
n3:klicoveSlovo
n12:Proteome n12:Protein%20reduction n12:Sulfur%20oxidation n12:Acidithiobacilus%20ferrooxidans n12:Iron%20oxidation
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[769E78EDC6B0]
n3:nazevZdroje
Proteomics
n3:obor
n15:CE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n19:GA525%2F04%2F1309
n3:rokUplatneniVysledku
n16:2006
n3:svazekPeriodika
6
n3:tvurceVysledku
Bořilová, Šárka Hallberg, Kevin B. Bouchal, Pavel Mandl, Martin Zdráhal, Zbyněk Janiczek, Oldřich
n3:zamer
n5:MSM0021622415 n5:MSM0021622413
s:issn
1615-9853
s:numberOfPages
8
n13:organizacniJednotka
14310