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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F06%3A00015708%21RIV10-MSM-14310___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
The purple pigmented bacterium Chromobacterium Violaceum is a dominant component of tropical soil microbiota that can cause rare but fatal septicaemia in humans. Its sequenced genome provides insight into the abundant potential of this organism for biotechnological and pharmaceutical applications and allowed an ORF encoding a protein that is 60% identical to the fucose binding lectin (PA-IIL) from Pseudomonas aeruginosa and the mannose binding lectin (RS-IIL) from Ralstonia solanacearum to be identified. The lectin, CV-IIL, has recently been purified from C. Violaceum [Zinger-Yosovich, K., Sudakevitz, D., Imberty, A., Garber, N. C., and Gilboa-Garber, N. (2006) Microbiology 152, 457-463] and has been confirmed to be a tetramer with subunit size of 11.86 kDa and a binding preference for fucose. We describe here the cloning of CV-IIL and its expression as a recombinant protein. The purple pigmented bacterium Chromobacterium Violaceum is a dominant component of tropical soil microbiota that can cause rare but fatal septicaemia in humans. Its sequenced genome provides insight into the abundant potential of this organism for biotechnological and pharmaceutical applications and allowed an ORF encoding a protein that is 60% identical to the fucose binding lectin (PA-IIL) from Pseudomonas aeruginosa and the mannose binding lectin (RS-IIL) from Ralstonia solanacearum to be identified. The lectin, CV-IIL, has recently been purified from C. Violaceum [Zinger-Yosovich, K., Sudakevitz, D., Imberty, A., Garber, N. C., and Gilboa-Garber, N. (2006) Microbiology 152, 457-463] and has been confirmed to be a tetramer with subunit size of 11.86 kDa and a binding preference for fucose. We describe here the cloning of CV-IIL and its expression as a recombinant protein.
dcterms:title
Unusual Entropy Driven Affinity of Chromobacterium violaceum Lectin CV-IIL towards Fucose and Mannose Unusual Entropy Driven Affinity of Chromobacterium violaceum Lectin CV-IIL towards Fucose and Mannose
skos:prefLabel
Unusual Entropy Driven Affinity of Chromobacterium violaceum Lectin CV-IIL towards Fucose and Mannose Unusual Entropy Driven Affinity of Chromobacterium violaceum Lectin CV-IIL towards Fucose and Mannose
skos:notation
RIV/00216224:14310/06:00015708!RIV10-MSM-14310___
n3:aktivita
n8:P n8:Z
n3:aktivity
P(GD204/03/H016), Z(MSM0021622413)
n3:cisloPeriodika
24
n3:dodaniDat
n15:2010
n3:domaciTvurceVysledku
n14:7374062 n14:5616697 n14:7678037 n14:5522064
n3:druhVysledku
n4:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
505097
n3:idVysledku
RIV/00216224:14310/06:00015708
n3:jazykVysledku
n16:eng
n3:klicovaSlova
lectin; microcalorimetry; chromobacterium violaceum; pathogen
n3:klicoveSlovo
n7:lectin n7:microcalorimetry n7:pathogen n7:chromobacterium%20violaceum
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7FFD5056EC34]
n3:nazevZdroje
Biochemistry
n3:obor
n10:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
10
n3:projekt
n17:GD204%2F03%2FH016
n3:rokUplatneniVysledku
n15:2006
n3:svazekPeriodika
45
n3:tvurceVysledku
Gilboa-Garber, Nechama Kostlánová, Nikola Wimmerová, Michaela Adam, Jan Rebuffet, Etienne Perret, Stephanie Imberty, Anne Mitchell, Edward P. Pokorná, Martina Ciocci, Gianluca
n3:zamer
n9:MSM0021622413
s:issn
0006-2960
s:numberOfPages
10
n19:organizacniJednotka
14310