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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F05%3A00014629%21RIV10-MSM-14310___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found.
dcterms:title
Elimination voltammetry with linear scan as a new detection method for DNA sensors Elimination voltammetry with linear scan as a new detection method for DNA sensors
skos:prefLabel
Elimination voltammetry with linear scan as a new detection method for DNA sensors Elimination voltammetry with linear scan as a new detection method for DNA sensors
skos:notation
RIV/00216224:14310/05:00014629!RIV10-MSM-14310___
n4:aktivita
n14:P n14:Z
n4:aktivity
P(1H-PK/42), P(GP525/04/P132), Z(AV0Z50040507), Z(MSM0021622412)
n4:cisloPeriodika
6-10
n4:dodaniDat
n16:2010
n4:domaciTvurceVysledku
n12:4995775 n12:2307049 n12:6563902 n12:3790185 n12:4637844
n4:druhVysledku
n15:J
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
520102
n4:idVysledku
RIV/00216224:14310/05:00014629
n4:jazykVysledku
n9:eng
n4:klicovaSlova
oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides
n4:klicoveSlovo
n5:sequence%20of%20nucleotides n5:DNA%20biosensor n5:elimination%20voltammetry%20with%20linear%20scan n5:linear%20sweep%20voltammetry n5:mercury%20electrode n5:reduction%20of%20adenine%20and%20cytosine n5:oligodeoxynucleotide
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[F9B2A8F6B28A]
n4:nazevZdroje
SENSORS
n4:obor
n8:CG
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
6
n4:projekt
n7:GP525%2F04%2FP132 n7:1H-PK%2F42
n4:rokUplatneniVysledku
n16:2005
n4:svazekPeriodika
5
n4:tvurceVysledku
Adam, Vojtěch Kizek, René Trnková, Libuše Jelen, František Potěšil, David Petrlová, Jitka
n4:zamer
n6:MSM0021622412 n6:AV0Z50040507
s:issn
1424-8220
s:numberOfPages
17
n11:organizacniJednotka
14310