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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F05%3A00014346%21RIV10-MSM-14310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min.
dcterms:title
Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
skos:prefLabel
Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
skos:notation
RIV/00216224:14310/05:00014346!RIV10-MSM-14310___
n4:aktivita
n10:Z
n4:aktivity
Z(MSM0021622413)
n4:cisloPeriodika
23
n4:dodaniDat
n9:2010
n4:domaciTvurceVysledku
n18:6669719 n18:7101813
n4:druhVysledku
n15:J
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
511861
n4:idVysledku
RIV/00216224:14310/05:00014346
n4:jazykVysledku
n17:eng
n4:klicovaSlova
electrochemical biosensor; enzyme electrode; phenol; catechol; waste water
n4:klicoveSlovo
n6:catechol n6:phenol n6:electrochemical%20biosensor n6:enzyme%20electrode n6:waste%20water
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[48C1A83542AB]
n4:nazevZdroje
Electroanalysis
n4:obor
n8:CE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:rokUplatneniVysledku
n9:2005
n4:svazekPeriodika
17
n4:tvurceVysledku
Skládal, Petr Solná, Renáta
n4:zamer
n5:MSM0021622413
s:issn
1040-0397
s:numberOfPages
10
n14:organizacniJednotka
14310