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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F05%3A00013672%21RIV10-MSM-14310___
rdf:type
n4:Vysledek skos:Concept
dcterms:description
3-Aminophenylboronic acid (APBA) was used for construction of affinity mass sensors for determination of saccharides and glycated hemoglobin using complexation reaction with diol groups. Two approaches were tested for the bioligand layer fabrication-incorporation of APBA inside a thicker matrix and immobilization as a self-assembled monolayer, respectively. The direct affinity sensor with APBA embeded in the structure of glutaraldehyde-crosslinked bovine serum albumine provided linear response to mono- and dissacharides in the range from 0.1 to 15.0 mg/ml. This biosensor was also used for kinetic analysis of the interaction of boronic acid with diols; the values of association and dissociation constants were determined. The sensors with a monolayer of boronic groups were better suited for binding of glycated hemoglobin, probably due to improved steric access to the ligand. 3-Aminophenylboronic acid (APBA) was used for construction of affinity mass sensors for determination of saccharides and glycated hemoglobin using complexation reaction with diol groups. Two approaches were tested for the bioligand layer fabrication-incorporation of APBA inside a thicker matrix and immobilization as a self-assembled monolayer, respectively. The direct affinity sensor with APBA embeded in the structure of glutaraldehyde-crosslinked bovine serum albumine provided linear response to mono- and dissacharides in the range from 0.1 to 15.0 mg/ml. This biosensor was also used for kinetic analysis of the interaction of boronic acid with diols; the values of association and dissociation constants were determined. The sensors with a monolayer of boronic groups were better suited for binding of glycated hemoglobin, probably due to improved steric access to the ligand.
dcterms:title
Quartz crystal biosensor for detection of sugars and glycated hemoglobin Quartz crystal biosensor for detection of sugars and glycated hemoglobin
skos:prefLabel
Quartz crystal biosensor for detection of sugars and glycated hemoglobin Quartz crystal biosensor for detection of sugars and glycated hemoglobin
skos:notation
RIV/00216224:14310/05:00013672!RIV10-MSM-14310___
n3:aktivita
n17:Z
n3:aktivity
Z(MSM0021622413)
n3:cisloPeriodika
1
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n10:6669719 n10:3133745
n3:druhVysledku
n18:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
539865
n3:idVysledku
RIV/00216224:14310/05:00013672
n3:jazykVysledku
n6:eng
n3:klicovaSlova
quartz crystal microbalance; m-aminophenylboronic acid; saccharides; glycated hemoglobin; kinetic analysis
n3:klicoveSlovo
n9:glycated%20hemoglobin n9:quartz%20crystal%20microbalance n9:saccharides n9:kinetic%20analysis n9:m-aminophenylboronic%20acid
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[1773141FF5CB]
n3:nazevZdroje
Analytica Chimica Acta
n3:obor
n13:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n11:2005
n3:svazekPeriodika
530
n3:tvurceVysledku
Přibyl, Jan Skládal, Petr
n3:zamer
n14:MSM0021622413
s:issn
0003-2670
s:numberOfPages
10
n8:organizacniJednotka
14310