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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F04%3A00011235%21RIV08-AV0-14310___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The group cysteine-containing peptides (metallothioneines, phytochelatins, and glutathione) plays an important role in active homeostasis and detoxification of metals in animal, plant, and/or microorganism cells. In this study we describe a novel approach to electroanalysis of cys-containing peptides and proteins. This approach is based on catalytic processes (presodium wave, peak H, Brdicka current) proceeding at negative potentials (ca. 1.8 V, Ag/AgCl/3M KCl) on the hanging mercury drop electrode (HMDE). A presodium wave is current-potential response accompanied with catalytic hydrogen evolution from aqueous solutions in the presence of proteins. It was found that the sulfhydryl(SH) groups in proteins are responsible for presodium catalytic processes. The highest sensitivity in the determination was observed with derivative chronopotentiometric stripping analysis (CPSA), which monitored the catalytic signal known as peak H. The determination of the low content of Cys-peptides was successfully perfor Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod. The group cysteine-containing peptides (metallothioneines, phytochelatins, and glutathione) plays an important role in active homeostasis and detoxification of metals in animal, plant, and/or microorganism cells. In this study we describe a novel approach to electroanalysis of cys-containing peptides and proteins. This approach is based on catalytic processes (presodium wave, peak H, Brdicka current) proceeding at negative potentials (ca. 1.8 V, Ag/AgCl/3M KCl) on the hanging mercury drop electrode (HMDE). A presodium wave is current-potential response accompanied with catalytic hydrogen evolution from aqueous solutions in the presence of proteins. It was found that the sulfhydryl(SH) groups in proteins are responsible for presodium catalytic processes. The highest sensitivity in the determination was observed with derivative chronopotentiometric stripping analysis (CPSA), which monitored the catalytic signal known as peak H. The determination of the low content of Cys-peptides was successfully perfor
dcterms:title
Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod
skos:prefLabel
Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod
skos:notation
RIV/00216224:14310/04:00011235!RIV08-AV0-14310___
n4:strany
431 - 431
n4:aktivita
n12:P
n4:aktivity
P(GA203/02/0422), P(IAA1163201)
n4:dodaniDat
n10:2008
n4:domaciTvurceVysledku
n5:4637844 n5:4995775 n5:3790185
n4:druhVysledku
n9:D
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
562506
n4:idVysledku
RIV/00216224:14310/04:00011235
n4:jazykVysledku
n19:eng
n4:klicovaSlova
metallothioneines; phytochelatins; glutathione; mercury electrode; catalytic reactions; Brdicka reaction; peak H
n4:klicoveSlovo
n8:metallothioneines n8:phytochelatins n8:Brdicka%20reaction n8:catalytic%20reactions n8:glutathione n8:peak%20H n8:mercury%20electrode
n4:kontrolniKodProRIV
[5FBFD2E3DA67]
n4:mistoKonaniAkce
Thessaloniki, Greece
n4:mistoVydani
Thessaloniki, Greece
n4:nazevZdroje
Book of Abstracts - 55th Annual Meeting of the ISE
n4:obor
n15:CG
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n14:GA203%2F02%2F0422 n14:IAA1163201
n4:rokUplatneniVysledku
n10:2004
n4:tvurceVysledku
Vacek, Jan Jelen, František Kizek, René Trnková, Libuše
n4:typAkce
n18:WRD
n4:zahajeniAkce
2004-02-19+01:00
s:numberOfPages
1
n20:hasPublisher
International Society of Electrochemistry
n6:organizacniJednotka
14310