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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F04%3A00010926%21RIV08-MSM-14310___
rdf:type
n17:Vysledek skos:Concept
dcterms:description
Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics, particularly due to resemblance of its aerobic respiratory chain to that of mitochondria. Also an aspect of a great nutritional adaptability was discovered, related to the ability of exploiting various electron donors and electron acceptors for maintenance and growth. To discuss mechanisms underlying regulation of gene expression by growth conditions, a high-throughput proteome mapping is one of the most effective tools to-date. For 2-D electrophoretic analysis of whole-cell extract and its membrane fraction, isoelectric focusation with immobilized pH gradients in the first dimension and SDS-PAGE in the second dimension were used. Using this approach, more than 800 protein spots of total cell extract and membrane fraction were detected in the range pI 3-10 and Mr 15-100 kDa. We compared complex protein composition of whole cells of P. denitrificans cultivated under aero Proteomová analýza bakterie Paracoccus denitrificans v závislosti na použitém terminálním akceptoru elektronů a přítomnosti azidu. Jedná se o předběžnou prezentaci proteomové studie, která byla následně publikována: Bouchal P., Přecechtělová P., Zdráhal Z., Kučera I.: Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide. Proteomics 2004, 4, 2662-2671. Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics, particularly due to resemblance of its aerobic respiratory chain to that of mitochondria. Also an aspect of a great nutritional adaptability was discovered, related to the ability of exploiting various electron donors and electron acceptors for maintenance and growth. To discuss mechanisms underlying regulation of gene expression by growth conditions, a high-throughput proteome mapping is one of the most effective tools to-date. For 2-D electrophoretic analysis of whole-cell extract and its membrane fraction, isoelectric focusation with immobilized pH gradients in the first dimension and SDS-PAGE in the second dimension were used. Using this approach, more than 800 protein spots of total cell extract and membrane fraction were detected in the range pI 3-10 and Mr 15-100 kDa. We compared complex protein composition of whole cells of P. denitrificans cultivated under aero
dcterms:title
Vliv terminálního akceptoru elektronů a azidu na proteinové složení bakterie Paracoccus denitrificans The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition
skos:prefLabel
The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition The effects of terminal electron acceptor and sodium azide on the Paracoccus denitrificans protein composition Vliv terminálního akceptoru elektronů a azidu na proteinové složení bakterie Paracoccus denitrificans
skos:notation
RIV/00216224:14310/04:00010926!RIV08-MSM-14310___
n3:strany
28-29
n3:aktivita
n5:P n5:Z
n3:aktivity
P(GA203/01/1589), Z(MSM 143100005), Z(MSM 143100008)
n3:dodaniDat
n6:2008
n3:domaciTvurceVysledku
n16:4568567 n16:3087719 n16:2503786 n16:9011978
n3:druhVysledku
n11:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
562200
n3:idVysledku
RIV/00216224:14310/04:00010926
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Paracoccus denitrificans; proteome analysis; terminal electron acceptor; azide
n3:klicoveSlovo
n4:proteome%20analysis n4:Paracoccus%20denitrificans n4:azide n4:terminal%20electron%20acceptor
n3:kontrolniKodProRIV
[6A58C1F95232]
n3:mistoKonaniAkce
31.8.-3.9.2004
n3:mistoVydani
Olomouc
n3:nazevZdroje
Acta Universitatis Palackianae Olomucensis - Chemica 43S
n3:obor
n9:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n8:GA203%2F01%2F1589
n3:rokUplatneniVysledku
n6:2004
n3:tvurceVysledku
Zdráhal, Zbyněk Přecechtělová, Petra Kučera, Igor Bouchal, Pavel
n3:typAkce
n10:EUR
n3:zahajeniAkce
2004-01-01+01:00
n3:zamer
n18:MSM%20143100008 n18:MSM%20143100005
s:numberOfPages
2
n15:hasPublisher
Univerzita Palackého v Olomouci
n21:isbn
80-244-0882-1
n22:organizacniJednotka
14310