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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F04%3A00010788%21RIV%2F2005%2FMSM%2F143105%2FN
rdf:type
skos:Concept n11:Vysledek
dcterms:description
One of the possible approaches to a plant gene function study in model plant Arabidopsis thaliana leads across T-DNA mutagenesis. Collection of 2500 T-DNA lines have been obtained by A. thaliana transformation with Agrobacterium tumefaciens containing plasmid pPCVRN4 with HPT selectable gene (Koncz et al. 1994). Screening of individual lines revealed three different mutations with defects in immature seed development (marked 1265, 1293, 1321) which have been characterised and analysed in detail. One T-DNA insert was determined in each line and the mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. Morphological studies of embryo development were performed by clearing in chloral hydrate. Mutant embryos were blocked in certain steps in the process necessary for embryo viability and development. In 1265 mutation defect in globular stage was observed. It was associated with abnormal pattern of cell division connected with shoot apical meristem formation during embryogenesi One of the possible approaches to a plant gene function study in model plant Arabidopsis thaliana leads across T-DNA mutagenesis. Collection of 2500 T-DNA lines have been obtained by A. thaliana transformation with Agrobacterium tumefaciens containing plasmid pPCVRN4 with HPT selectable gene (Koncz et al. 1994). Screening of individual lines revealed three different mutations with defects in immature seed development (marked 1265, 1293, 1321) which have been characterised and analysed in detail. One T-DNA insert was determined in each line and the mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. Morphological studies of embryo development were performed by clearing in chloral hydrate. Mutant embryos were blocked in certain steps in the process necessary for embryo viability and development. In 1265 mutation defect in globular stage was observed. It was associated with abnormal pattern of cell division connected with shoot apical meristem formation during embryogenesi Jedním z možných přístupů studia funkcí rostlinných genů u Arabidopsis thaliana je T-DNA mutageneze. Kolekce 2500 T-DNA linií po transformaci Agrobacterium tumefaciens byla využita při vyhledávání mutací s embryonálními defekty. Tři tyto mutace (1265, 1293, 1321) podrobně analyzovány a charakterizovány. Genetická analýza potvrdila monogenní a recesivní charakter těchto mutací.
dcterms:title
Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana. Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana. Identifikace a characterizace tří genů determinujících embryogenezi prostřednictvím T-DNA mutací u Arabidopsis thaliana.
skos:prefLabel
Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana. Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana. Identifikace a characterizace tří genů determinujících embryogenezi prostřednictvím T-DNA mutací u Arabidopsis thaliana.
skos:notation
RIV/00216224:14310/04:00010788!RIV/2005/MSM/143105/N
n3:strany
T01-037
n3:aktivita
n9:Z
n3:aktivity
Z(MSM 143100008)
n3:dodaniDat
n12:2005
n3:domaciTvurceVysledku
n8:6283853 n8:5004209
n3:druhVysledku
n19:D
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
567128
n3:idVysledku
RIV/00216224:14310/04:00010788
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Arabidopsis thaliana, T-DNA
n3:klicoveSlovo
n17:Arabidopsis%20thaliana n17:T-DNA
n3:kontrolniKodProRIV
[3870D9796B66]
n3:mistoKonaniAkce
Berlin, Germany
n3:mistoVydani
Berlin, Germany
n3:nazevZdroje
Abstract Book of the 15th International Conference on Arabidopsis Research, July 11-14, Berlin, Germany.
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n12:2004
n3:tvurceVysledku
Kocábek, Tomáš Dudová, Markéta Řepková, Jana
n3:typAkce
n14:WRD
n3:zahajeniAkce
2004-07-11+02:00
n3:zamer
n13:MSM%20143100008
s:numberOfPages
1
n4:hasPublisher
Max Planck Institute of Molecular Plant Physiology
n20:organizacniJednotka
14310