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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F04%3A00010413%21RIV09-GA0-14310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGE with subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes Bakteriofág 812, zástupce čeledi Myoviridae, je virulentní polyvalentní fág s �irokým rozmezím hostitelů, zahrnujícím kmeny a druhy rodu Staphylococcus. Tyto vlastnosti jej činí vhodným kandidátem pro fágovou terapii stafylokokových infekcí. Tato práce byla zaměřena na srovnání genomu a proteomu standardního typu fága 812, jeho mutant v rozmezí hostitele a příbuzných fágů U16, SK311, 131 a Twort s cílem objastnit molekulární základ jejich rozdílného hostitelského rozmezí. Genomy uvedených fágů tvořené lineární dvouřetězcovou DNA o velikosti 145 kb byly srovnávány pomocí restrikční analýzy. Úseky vykazující u jednotlivých fágů rozdíly byly pak charakterizovány detailně. Pro účely proteomických studií byla pou�ita 1-D SDS-PAGE. Nalezené proteiny byly identifikovány metodou peptidového mapování s vyu�itím MALDI-TOF hmotnostní spektrometrie. Srovnáním fágových genomů byly zji�těny vedle nukleotidových substitucí té� rozsáhlej�í přestavby sekvencí DNA. Inzerce a delece o velikosti ~1 a� 3 kb byly nalezeny Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGE with subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes
dcterms:title
Genomic and proteomic characterization of the polyvalent staphylophage 812 Genomic and proteomic characterization of the polyvalent staphylophage 812 Charakterizace genomu a proteomu polyvalentního fága 812
skos:prefLabel
Charakterizace genomu a proteomu polyvalentního fága 812 Genomic and proteomic characterization of the polyvalent staphylophage 812 Genomic and proteomic characterization of the polyvalent staphylophage 812
skos:notation
RIV/00216224:14310/04:00010413!RIV09-GA0-14310___
n4:aktivita
n11:Z n11:P
n4:aktivity
P(GA203/03/0515), Z(MSM 143100008)
n4:dodaniDat
n10:2009
n4:domaciTvurceVysledku
n6:9725741 n6:3440656 n6:9413359 n6:9236333 n6:4186672 n6:4568567 n6:3431525 n6:5051207
n4:druhVysledku
n18:D
n4:duvernostUdaju
n9:S
n4:entitaPredkladatele
n21:predkladatel
n4:idSjednocenehoVysledku
565400
n4:idVysledku
RIV/00216224:14310/04:00010413
n4:jazykVysledku
n17:eng
n4:klicovaSlova
staphylococcus; bacteriophage
n4:klicoveSlovo
n5:staphylococcus n5:bacteriophage
n4:kontrolniKodProRIV
[62E5C4C4F587]
n4:mistoKonaniAkce
Charleston, South Carolina, USA
n4:mistoVydani
Charleston, South Carolina, USA
n4:nazevZdroje
11th International Symposium on Staphylococci & Staphylococcal Infections. Plenary Summaries & Poster Abstracts
n4:obor
n15:EB
n4:pocetDomacichTvurcuVysledku
8
n4:pocetTvurcuVysledku
8
n4:projekt
n19:GA203%2F03%2F0515
n4:rokUplatneniVysledku
n10:2004
n4:tvurceVysledku
Pantůček, Roman Doškař, Jiří Růžičková, Vladislava Konečná, Hana Preisler, Jan Kašpárek, Petr Zdráhal, Zbyněk Eyer, Luděk
n4:typAkce
n14:WRD
n4:zahajeniAkce
2004-10-24+02:00
n4:zamer
n16:MSM%20143100008
s:numberOfPages
1
n20:hasPublisher
Medical University of South Carolina
n3:organizacniJednotka
14310