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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F04%3A00010230%21RIV08-MSM-14310___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
New specific primers AR1 and AR2 were successfully used for the amplification of a specific part of internal transcribed spacer (ITS) of rDNA of Armillaria isolated from soil samples.The individual species were distinguished by restriction fragment length polymorphisms (RFLP) analysis with restriction endonucleases HinfI. For the first time, a method is described enabling the identification of Armillaria species within one day directly from soil samples without the need for previous isolation and cultivation of mycelium New specific primers AR1 and AR2 were successfully used for the amplification of a specific part of internal transcribed spacer (ITS) of rDNA of Armillaria isolated from soil samples.The individual species were distinguished by restriction fragment length polymorphisms (RFLP) analysis with restriction endonucleases HinfI. For the first time, a method is described enabling the identification of Armillaria species within one day directly from soil samples without the need for previous isolation and cultivation of mycelium V rámci studie byly připraveny specifické primery AR1 a AR2, které slouží k amplifikaci specifické části oblasti ITS v rDNA václavek. Různé druhy václavek byly rozlišovány pomocí RFLP amplifikovaného fragmentu. Námi vyvinutá metoda umožňuje v průběhu jednoho dne určit druh václavky z půdního vzorku pomocí nested PCR a to bez zdlouhavé kultivace.
dcterms:title
Rychlá identifikace evropských druhů václavek z půdních vzorků pomocí metody nested PCR The rapid identification of European Armillaria species from soil samples by nested PCR The rapid identification of European Armillaria species from soil samples by nested PCR
skos:prefLabel
The rapid identification of European Armillaria species from soil samples by nested PCR The rapid identification of European Armillaria species from soil samples by nested PCR Rychlá identifikace evropských druhů václavek z půdních vzorků pomocí metody nested PCR
skos:notation
RIV/00216224:14310/04:00010230!RIV08-MSM-14310___
n3:strany
105
n3:aktivita
n18:Z
n3:aktivity
Z(MSM 143100005)
n3:cisloPeriodika
237
n3:dodaniDat
n14:2008
n3:domaciTvurceVysledku
n8:3881652 n8:9217088 n8:9713050
n3:druhVysledku
n13:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
583552
n3:idVysledku
RIV/00216224:14310/04:00010230
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Armillaria; PCR; soil
n3:klicoveSlovo
n9:soil n9:Armillaria n9:PCR
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[D75D763311DA]
n3:nazevZdroje
FEMS Microbiology Letters
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n14:2004
n3:svazekPeriodika
2004
n3:tvurceVysledku
Šerý, Omar Mikeš, Vladimír Lochman, Jan
n3:zamer
n11:MSM%20143100005
s:issn
0378-1097
s:numberOfPages
6
n17:organizacniJednotka
14310