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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F03%3A00008258%21RIV09-GA0-14310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Using slot-blot and fluorescent in situ hybridization (FISH), we found no evidence for the presence of the Arabidopsis-type telomeric sequence (TTTAGGG)n at the chromosome termini in any of the Cestrum species we investigated. Probing for the human-type telomere (TTAGGG)n also revealed no signal. However, polymerase chain reaction experiments indicated that there are short lengths of the sequence TTTAGGG dispersed in the genome but that these sequences are almost certainly too short to act as functional telomeres even if they were at the chromosome termini. An analysis of related genera Vestia and Sessea indicates that they too lack the Arabidopsis-type telomere, and the sequences were lost in the common ancestor of these genera. We found that the Cestrum species investigated had particularly large mean chromosome sizes. We discuss whether this is a consequence of alternative telomere end maintenance systems. Using slot-blot and fluorescent in situ hybridization (FISH), we found no evidence for the presence of the Arabidopsis-type telomeric sequence (TTTAGGG)n at the chromosome termini in any of the Cestrum species we investigated. Probing for the human-type telomere (TTAGGG)n also revealed no signal. However, polymerase chain reaction experiments indicated that there are short lengths of the sequence TTTAGGG dispersed in the genome but that these sequences are almost certainly too short to act as functional telomeres even if they were at the chromosome termini. An analysis of related genera Vestia and Sessea indicates that they too lack the Arabidopsis-type telomere, and the sequences were lost in the common ancestor of these genera. We found that the Cestrum species investigated had particularly large mean chromosome sizes. We discuss whether this is a consequence of alternative telomere end maintenance systems. Using slot-blot and fluorescent in situ hybridization (FISH), we found no evidence for the presence of the Arabidopsis-type telomeric sequence (TTTAGGG)n at the chromosome termini in any of the Cestrum species we investigated. Probing for the human-type telomere (TTAGGG)n also revealed no signal. However, polymerase chain reaction experiments indicated that there are short lengths of the sequence TTTAGGG dispersed in the genome but that these sequences are almost certainly too short to act as functional telomeres even if they were at the chromosome termini. An analysis of related genera Vestia and Sessea indicates that they too lack the Arabidopsis-type telomere, and the sequences were lost in the common ancestor of these genera. We found that the Cestrum species investigated had particularly large mean chromosome sizes. We discuss whether this is a consequence of alternative telomere end maintenance systems.
dcterms:title
The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots
skos:prefLabel
The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots
skos:notation
RIV/00216224:14310/03:00008258!RIV09-GA0-14310___
n4:aktivita
n13:P n13:Z
n4:aktivity
P(GA204/02/0027), Z(AV0Z5004920), Z(MSM 143100008)
n4:cisloPeriodika
34
n4:dodaniDat
n14:2009
n4:domaciTvurceVysledku
n7:9937870 n7:8202575
n4:druhVysledku
n10:J
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
597273
n4:idVysledku
RIV/00216224:14310/03:00008258
n4:jazykVysledku
n6:eng
n4:klicovaSlova
absence of TTTAGGG telomere; dicot; chromosome; FISH
n4:klicoveSlovo
n9:chromosome n9:dicot n9:absence%20of%20TTTAGGG%20telomere n9:FISH
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[03433BD60AF5]
n4:nazevZdroje
Plant Journal
n4:obor
n5:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:projekt
n19:GA204%2F02%2F0027
n4:rokUplatneniVysledku
n14:2003
n4:svazekPeriodika
Neuveden
n4:tvurceVysledku
Leitch, Andrew Rowland Lim, Kar Yoong Leitch, Ilia Judith Sýkorová, Eva Fajkus, Jiří Chase, Mark W. Knapp, Sandra
n4:wos
000182455200003
n4:zamer
n11:MSM%20143100008 n11:AV0Z5004920
s:issn
0960-7412
s:numberOfPages
9
n17:organizacniJednotka
14310