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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F03%3A00008145%21RIV08-GA0-14310___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells. Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells. Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells.
dcterms:title
Immunomagnetic separation of Salmonella cells using newly designed carriers Immunomagnetic separation of Salmonella cells using newly designed carriers Immunomagnetic separation of Salmonella cells using newly designed carriers
skos:prefLabel
Immunomagnetic separation of Salmonella cells using newly designed carriers Immunomagnetic separation of Salmonella cells using newly designed carriers Immunomagnetic separation of Salmonella cells using newly designed carriers
skos:notation
RIV/00216224:14310/03:00008145!RIV08-GA0-14310___
n3:strany
215-221
n3:aktivita
n17:P
n3:aktivity
P(GA203/00/1339)
n3:cisloPeriodika
1-2
n3:dodaniDat
n11:2008
n3:domaciTvurceVysledku
n8:4977459 n8:9327711 n8:6416691 n8:2412594
n3:druhVysledku
n14:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
609838
n3:idVysledku
RIV/00216224:14310/03:00008145
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Salmonella spp. magnetic sorbents; immobilization; affinity sorbents; hydroxyethyl methacrylate; glycidyl methacrylate; ethylene methacrylate; proteinase K; enzymes; antibodies
n3:klicoveSlovo
n4:proteinase%20K n4:hydroxyethyl%20methacrylate n4:antibodies n4:ethylene%20methacrylate n4:glycidyl%20methacrylate n4:enzymes n4:Salmonella%20spp.%20magnetic%20sorbents n4:immobilization n4:affinity%20sorbents
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[70C9CF640895]
n3:nazevZdroje
Journal of Chromatography A
n3:obor
n15:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
7
n3:projekt
n16:GA203%2F00%2F1339
n3:rokUplatneniVysledku
n11:2003
n3:svazekPeriodika
1009
n3:tvurceVysledku
Španová, Alena Rittich, Bohuslav Sučiková, Jana Štrumcová, Soňa Prodělalová, Jana Horák, Daniel Lenfeld, Jiří
s:issn
0021-9673
s:numberOfPages
7
n18:organizacniJednotka
14310