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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F03%3A00008100%21RIV%2F2004%2FGA0%2F143104%2FN
rdf:type
n18:Vysledek skos:Concept
dcterms:description
The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. Genetic engineering has been previously applied for obtaining phage mutants with the desired host specificity. However, the genomic sequence similarity of the different phages is generally low, and identification of proteins with the same function in various phages based solely on the DNA sequence is often impossible. Furthermore, amino acid modifications, such as variants and post-translational modifications of the predicted proteins cannot be deduced from the DNA sequence. In this work, we propose new approaches to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis. Additionally, The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. Genetic engineering has been previously applied for obtaining phage mutants with the desired host specificity. However, the genomic sequence similarity of the different phages is generally low, and identification of proteins with the same function in various phages based solely on the DNA sequence is often impossible. Furthermore, amino acid modifications, such as variants and post-translational modifications of the predicted proteins cannot be deduced from the DNA sequence. In this work, we propose new approaches to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis. Additionally,
dcterms:title
Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry
skos:prefLabel
Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry
skos:notation
RIV/00216224:14310/03:00008100!RIV/2004/GA0/143104/N
n3:strany
1152
n3:aktivita
n17:P n17:Z
n3:aktivity
P(GA203/03/0515), Z(MSM 143100008)
n3:dodaniDat
n15:2004
n3:domaciTvurceVysledku
n12:9236333 n12:5051207 n12:8857741 n12:3254658 n12:3440656 n12:4186672 n12:4568567 n12:9725741 n12:2941406
n3:druhVysledku
n11:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
623992
n3:idVysledku
RIV/00216224:14310/03:00008100
n3:jazykVysledku
n9:eng
n3:klicovaSlova
MALDI; staphylococcal bacteriophage; proteome
n3:klicoveSlovo
n8:staphylococcal%20bacteriophage n8:MALDI n8:proteome
n3:kontrolniKodProRIV
[56AD4FACC834]
n3:mistoKonaniAkce
Montreal, Canada
n3:mistoVydani
Montreal
n3:nazevZdroje
51st Annual Conference on Mass Spectrometry and Allied Topics - Abstract book
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
11
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n6:GA203%2F03%2F0515
n3:rokUplatneniVysledku
n15:2003
n3:tvurceVysledku
Malá, Zdena Zdráhal, Zbyněk Klepárník, Karel Krásenský, Pavel Růžičková, Vladislava Konečná, Hana Foret, František Doškař, Jiří Vrábel, Patrik Preisler, Jan Pantůček, Roman
n3:typAkce
n5:WRD
n3:zahajeniAkce
2003-06-08+02:00
n3:zamer
n16:MSM%20143100008
s:numberOfPages
1
n14:hasPublisher
American Society for Mass Spectrometry
n20:organizacniJednotka
14310